Methylene blue usage in the evaluation of traumatic necrosis in a rabbit liver injury model

Behdad, A. and Hosseinpour, M. (2009) Methylene blue usage in the evaluation of traumatic necrosis in a rabbit liver injury model. Ulusal Travma ve Acil Cerrahi Dergisi, 15 (4). pp. 337-341. ISSN 1306-696X


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BACKGROUND: In this study, the efficacy of methylene blue in the differentiation of devascularized and vital areas in a traumatic liver model in rabbits was investigated. METHODS: Twenty healthy rabbits were selected and randomly divided into two equal groups. Surgery was performed through an upper abdominal mid-line incision 5 cm in length. After induction of liver injury, diluted methylene blue was injected through the portal vein and staining onset and discoloration times were recorded. RESULTS: All 20 rabbits survived until the end of the experiment. In cases, non-perfused areas of the liver remained unstained. In control rabbits, liver parenchyma was stained homogeneously. The mean onset time of staining in normal parenchyma was 18.1±1.3 sec in cases and 17.7±1.4 sec in controls (p=NS). The mean discoloration time was 53.1±3.7 sec in cases and 53.1±3.2 sec in controls (p=NS). CONCLUSION: In major liver injuries requiring surgical intervention, methylene blue injection can be used for detection of non-perfused areas in the liver.

Item Type: Article
Additional Information: cited By 2
Uncontrolled Keywords: alanine aminotransferase; aspartate aminotransferase; bilirubin; creatinine; methylene blue; urea, alanine aminotransferase blood level; animal experiment; animal model; article; aspartate aminotransferase blood level; bilirubin blood level; color; controlled study; creatinine blood level; experimental rabbit; kidney function test; liver function test; liver injury; liver necrosis; liver parenchyma; liver perfusion; methemoglobinemia; nonhuman; staining; urea blood level, Animals; Disease Models, Animal; Liver; Methylene Blue; Necrosis; Rabbits; Random Allocation; Staining and Labeling
Subjects: Medicine
Divisions: Faculty of Medicine > Clinical Sciences > Department of Surgery
Depositing User: editor . 2
Date Deposited: 28 Jan 2017 14:07
Last Modified: 28 Jan 2017 14:07

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