Molecular epidemiology of aminoglycosides resistance in acinetobacter spp. with emergence of multidrug-resistant strains

Moniri, R. and Kheltabadi-Farahani, R. and Shajari, G. and Nazem-Shirazi, M.H. and Ghasemi, A. (2010) Molecular epidemiology of aminoglycosides resistance in acinetobacter spp. with emergence of multidrug-resistant strains. Iranian Journal of Public Health, 39 (2). pp. 63-68.

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Background: Acinetobacter spp. is characterized as an important nosocomial pathogen and increasing antimicrobial resistance. Our aim was to evaluate antimicrobial susceptibility and aminoglycosides resistance genes of Acinetobacter spp. isolated from hospitalized patients. Methods: Sixty isolates were identified as Acinetobacter species. The isolates were tested for antibiotic resistance by disc diffusion method for 12 antimicrobials. The presence of aphA6, aacCl aadAl, and aadB genes were detected using PCR. Results: From the isolated Acinetobacter spp. the highest resistance rate showed against amikacin, tobramycin, and cef-tazidim, respectively; while isolated bacteria were more sensitive to ampicillic/subactam. More than 66 of the isolates were resistant to at least three classes of antibiotics, and 27.5 of MDR strains were resistant to all seven tested classes of antimicrobials. The higher MDR rate presented in bacteria isolated from the ICU and blood samples. More than 60 of the MDR bacteria were resistance to amikacin, ceftazidim, ciprofloxacin, piperacillin/tazobactam, doxycycline, tobramycin and levofloxacin. Also, more than 60 of the isolates contained phosphotransferase aphA6, and acetyltransferase genes aacC1, but adenylyltransferase genes aadA1 (41.7), and aadB (3.3) were less prominent. 21.7 of the strains contain three aminoglycoside resistance genes (aphA6, aacCl and aadAl). Conclusion: The rising trend of resistance to aminoglycosides poses an alarming threat to treatment of such infections. The findings showed that clinical isolates of Acinetobacter spp. in our hospital carrying various kinds of aminoglycoside resistance genes.

Item Type: Article
Additional Information: cited By 10
Subjects: Medicine
Immunology and Microbiology
Divisions: Faculty of Medicine > Basic Sciences > Department of Microbiology & Immunology
Depositing User: editor . 2
Date Deposited: 26 Feb 2017 08:25
Last Modified: 08 Mar 2017 23:20

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