Mirhashemi, S.M. and Moshtaghie, A.A. and Ani, M. and Aarabi, M.H. (2010) Lead toxicity on kinetic behaviors of high and low molecular weight alkaline phosphatase isoenzymes of rat, in vivo and in vitro studies. Journal of Biological Sciences, 10 (4). pp. 341-347.
Full text not available from this repository.Abstract
The relationship between lead (Pb) toxicity and changes in the kinetic characteristics of serum, liver and brain high and low molecular weight alkaline phosphatase isoenzymes has been examined in this document. Alkaline phosphatase is a family of phosphomonoesterases that was measured in serum, liver and brain using paranitrophenol phosphate (pNPP) as substrate and 2-amino-2-methyl-l-propanol as buffer. Protein concentration was determined as described by Bradford. Results obtained showed that every other day intrapritoneally injection of 39.5 ug kg-1 of lead as (Pb (CH3COO)2 3H20), in male rats for 2 consecutive weeks resulted in decreasing level of liver and brain alkaline phosphatase by 16.7 and 10.9, respectively, whereas an elevation of serum enzyme activity by 28.4 was seen in comparison to untreated controls (p<0.05). Long-term exposure to 13.2 ug kg-1 of this salt, showed a statistically significant reduction in liver and brain levels of alkaline phosphatase by 18.7 and 13.2 respectively and an increment in serum activity of the enzyme by 37.6 in compared to control group (p<0.05). Using gel filtration chromatography technique with sephacryl S300 showed that, in comparison to control groups, serum and liver homogenate from lead treated groups had a significant level of high molecular weight alkaline phosphatase, which might be considered as a potential biomarker for lead toxicity. In vitro experiments showed that lead inhibited all the isoenzymes. © 2010 Asian Network for Scientific Information.
| Item Type: | Article |
|---|---|
| Additional Information: | cited By 3 |
| Uncontrolled Keywords: | alkaline phosphatase isoenzyme; lead, animal experiment; article; brain; concentration response; controlled study; enzyme activity; exposure; gel filtration chromatography; homogenate; in vitro study; in vivo study; kinetics; liver; male; nonhuman; rat, Rattus |
| Subjects: | Medicine |
| Divisions: | Faculty of Medicine > Basic Sciences > Department of Biochemistry |
| Depositing User: | editor . 2 |
| Date Deposited: | 26 Feb 2017 09:44 |
| Last Modified: | 28 Feb 2017 15:01 |
| URI: | http://eprints.kaums.ac.ir/id/eprint/913 |
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