Study on ITS1 gene of Iranian Trichomonas vaginalis by molecular methods

Kazemi, F. and Hooshyar, H. and Zareikar, B. and Bandehpour, M. and Arbabi, M. and Talari, S.A. and Alizadeh, R. and Kazemi, B. (2010) Study on ITS1 gene of Iranian Trichomonas vaginalis by molecular methods. Iranian Journal of Parasitology, 5 (4). pp. 9-14.

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Background: Trichomoniasis is a worldwide protozoan parasitic disease and metronidazole is a choice drug for its treatment. Because of disease importance in public health and its controversial ideas about the prevalence of drug resistance, this study was carried out. Methods: Fifty-two suspected vaginal samples were collected from 2006 to 2007 in Gynecology Maryam Hospital, Tehran, Iran. All isolates were examined by microscopic, culture and PCR techniques. The PCR products were analyzed by RFLP and CSGE methods and two suspected samples were sequenced. Results: Trichomonas vaginalis was identified from all 52 samples. Of 52 isolates, 45 samples were successfully cultured and amplified by PCR except one. Seven were positive only by PCR. Finally, ITS1 fragment was successfully amplified in 51 of 52. CSGE analysis and PCR products digestion by MspI followed by sequencing showed nucleotide mutation at position 209 (C209T) of the ITS1 fragment in two (3.9) of them Conclusion: The results showed mutation in ITS1 fragment of T. vaginalis in two (3.9) of Iranian isolates which may be related to metronidazole resistance.

Item Type: Article
Additional Information: cited By 2
Uncontrolled Keywords: article; DNA extraction; female; gene; gene mutation; gene sequence; human; its1 gene; major clinical study; polymerase chain reaction; restriction fragment length polymorphism; Trichomonas vaginalis; trichomoniasis; vagina smear, Protozoa; Trichomonas vaginalis
Subjects: Medicine
Divisions: Faculty of Medicine > Basic Sciences > Department of Parasitological & Mycology
Depositing User: editor . 2
Date Deposited: 26 Feb 2017 09:55
Last Modified: 07 Jun 2017 14:06

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