Evaluation of Porin interaction with adenine nucleotide translocase and cyclophilin-D proteins after brain ischemia and reperfusion

Atlasi, M.A. and Velazquez, J.L.P. (2011) Evaluation of Porin interaction with adenine nucleotide translocase and cyclophilin-D proteins after brain ischemia and reperfusion. Iranian Journal of Basic Medical Sciences, 14 (1). pp. 17-24.

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Abstract

Objective (s) Porin is a mitochondrial outer membrane channel, which usually functions as the pathway for the movement of various substances in and out of the mitochondria and is considered to be a component of the permeability transition (PT) pore complex that plays a role in the PT. We addressed the hypothesis that porin interacts with other mitochondrial proteins after ischemic injury. Materials and Methods For this purpose, we used in vivo 4-vessel occlusion model of rat brain and porin purification method by hydroxyapatite column. After SDS gel electrophoresis and silver nitrate staining, Western blotting was done for porin, adenine nucleotide translocase and cyclophilin-D proteins. Results Porin was purified from mitochondrial mixture in ischemic brain and control groups. Investigation of interaction of adenine nucleotide transposes (ANT) and cyclophilin-D with porin by Western blotting showed no proteins co-purified with porin from injured tissues. Conclusion The present study implies that there may not be interaction between porin, and ANT or cyclophilin-D, and if there is any, it is not maintained during the purification procedure.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: adenine nucleotide translocase; cyclophilin D; porin, animal experiment; animal tissue; article; blood vessel occlusion; brain ischemia; brain perfusion; controlled study; immunoblotting; in vivo study; mitochondrial membrane; nonhuman; polyacrylamide gel electrophoresis; protein interaction; protein purification; rat; Western blotting, Rattus
Subjects: Anatomy Morphology
Divisions: Faculty of Medicine > Basic Sciences > Department of Anatomy
Depositing User: editor . 2
Date Deposited: 27 Feb 2017 04:27
Last Modified: 08 Mar 2017 21:38
URI: http://eprints.kaums.ac.ir/id/eprint/882

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