Detection and characterization of multidrug resistance and extended-spectrum-beta-lactamase-producing (ESBL s) pseudomonas aeruginosa isolates in teaching hospital

Tavajjohi, Z. and Moniri, R. and Khorshidi, A. (2011) Detection and characterization of multidrug resistance and extended-spectrum-beta-lactamase-producing (ESBL s) pseudomonas aeruginosa isolates in teaching hospital. African Journal of Microbiology Research, 5 (20). pp. 3223-3228.

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Abstract

Pseudomonas aeruginosa is the most common pathogen causing nosocomial infections. The objective of this study was to investigate the extended-spectrum-beta-lactamase (ESBLs) producing and multidrug resistance of hospital isolates of P. aeruginosa and to determine the presence of several resistance genes. A total of 86 isolates of P. aeruginosa were collected from teaching hospital in Kashan, Iran. Susceptibility to eight antimicrobial agents was performed by disk diffusion method. ESBL-phenotypic detection was carried out by double-disk synergy test; and the presence of the genes encoding of bla(TEM), bla(SHV), bla(CTX-M), bla(OXA) and bla(GES)-like genes was studied by polymerase chain reaction. The prevalence of ESBLs was 8.1. The presence of genes encoding ESBLs was confirmed in seven isolates, comprising seven bla(GES-2), one bla(SHV-1), one bla(SHV-5) and one bla(CTX-M-1) genes. P. aeruginosa demonstrated the highest resistance rate to piperacillin (38.4), 67.5 of isolates were sensitive to imipenem whereas 32.5 were MDR (resistant to three or more classes of antibiotics). A multidrug-resistant (MDR) phenotype occurred frequently in P. aeruginosa. bla(GES-2), which compromises the efficacy of imipenem detected in all of seven ESBLproducing P. aeruginosa strains. Proper infection control practices and barriers are essential to prevent spreading and outbreaks of ESBL-producing and MDR P. aeruginosa in our teaching hospital. © 2011 Academic Journals.

Item Type: Article
Additional Information: cited By 8
Uncontrolled Keywords: Pseudomonas aeruginosa
Subjects: Immunology and Microbiology
Divisions: Faculty of Medicine > Basic Sciences > Department of Microbiology & Immunology
Depositing User: editor . 2
Date Deposited: 27 Feb 2017 08:05
Last Modified: 08 Mar 2017 23:19
URI: http://eprints.kaums.ac.ir/id/eprint/836

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