To study various concentrations of magnesium and aluminium on amylin hormone conformation

Mirhashemi, S.M. and Aarabi, M.H. (2011) To study various concentrations of magnesium and aluminium on amylin hormone conformation. Pakistan Journal of Biological Sciences, 14 (11). pp. 653-657.

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Abstract

Type 2 diabetes mellitus can be defined as a conformational disease since a beta cell producing protein called amylin undergoes a change in the tertiary structure followed by self-aggregation and deposition. Amylin deposition causes destruction of pancreatic (3-cells. The aim of this study was to investigate whether different concentrations of magnesium and aluminium alter amylin conformation under near-physiological circumstances. Conformational variations were monitored by fluorescent method before and after incubation by shaker incubator in 37°C by LS55 spectrofluorometer instrument. This in vitro study showed that magnesium had contradictory effects on amylin folding and these effects were magnesium concentration dependent. Magnesium with concentration of 1 to 1.5 mM had inhibitoiy effect but in 2.5 to 3.5 mM promoted amylin misfolding significantly (p<0.05). The obtained data also demonstrated that aluminium with concentrations of 5, 10 and 20 juM had stimulatory effects on formation of beta-amyloid sheet significantly (p<0.05). It may be concluded that islet amyloid misfolding and cytotoxicity to 3-cells might be magnesium dose dependent in diabetic patients. © 2011 Asian Network for Scientific Information.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: aluminum; amylin; magnesium, article; chemistry; human; metabolism; non insulin dependent diabetes mellitus; protein conformation; protein folding, Aluminum; Diabetes Mellitus, Type 2; Humans; Islet Amyloid Polypeptide; Magnesium; Protein Conformation; Protein Folding
Subjects: Biochemistry, Genetics and Molecular Biology
Divisions: Faculty of Medicine > Basic Sciences > Department of Biochemistry
Depositing User: editor . 2
Date Deposited: 27 Feb 2017 08:45
Last Modified: 27 Feb 2017 08:45
URI: http://eprints.kaums.ac.ir/id/eprint/827

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