Distribution of gentamicin resistant genes of nosocomial enterococcus spp from intensive care unit of Shahid Beheshty Hospital in Kashan, Iran

Esmailzadeh, M. and Saffari, M. and Moniri, R. and Gilasi, H.R. and Jabbary, M. (2016) Distribution of gentamicin resistant genes of nosocomial enterococcus spp from intensive care unit of Shahid Beheshty Hospital in Kashan, Iran. Journal of Pure and Applied Microbiology, 10 (3). pp. 1919-1926.

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Abstract

The aim of this study was to determine, the rate and molecular characterization of aminoglycoside resistance genes (aac(6�)-Ie-aph(2�)-Ia, aph(2�)-Ib, aph(23)-Ic, and aph(23)-Id) among high level gentamicin resistance (HLGR) enterococcus isolates in Kashan, Iran. A total of 180 enterococcus species were tested for high level gentamicin resistance by using disk diffusion method and minimum inhibitory concentration (MIC>500 1/4g/mL) confirmatory test. High level gentamicin resistance strains were further assessed for aminoglycoside resistance genes. Antibiotic susceptibility pattern revealed that 43 isolates (23.9) were high level gentamicin resistance (HLGR) (MIC>5001/4g/ml), 24 isolates (55.8) of HLGR isolates were resistant to Chloramphenicol, 13 isolates (30.2) to Quinupristin-dalfopristin, 7 isolates (16.3) to Linezolid and 9 isolates (20.9) of HLGR isolates were multi-drug resistant. The PCR method revealed that 76.7 of high level gentamicin resistance isolates carried aac(62)Ie-aph(23)Ia gene; but aph(23)Ib, aph(23)Ic, and aph(23)Id genes were not detected among our isolates. The aac (6�)-Ie-aph (2�)-Ia was detected in (71.9) and (28.1), of Enterococcus faecalis and E. faecium, respectively. These results point to that high level aminoglycoside resistance genes are extensively disseminated among ICU isolates of enterococci.

Item Type: Article
Additional Information: cited By 0
Subjects: Immunology and Microbiology
Divisions: Faculty of Medicine > Basic Sciences > Department of Microbiology & Immunology
Depositing User: editor . 2
Date Deposited: 04 Mar 2017 08:02
Last Modified: 04 Mar 2017 08:02
URI: http://eprints.kaums.ac.ir/id/eprint/78

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