Neuroprotective properties of Melissa officinalis after hypoxic-ischemic injury both in vitro and in vivo

Bayat, M. and Azami-Tameh, A. and Hossein Ghahremani, M. and Akbari, M. and Mehr, S.E. and Khanavi, M. and Hassanzadeh, G. (2012) Neuroprotective properties of Melissa officinalis after hypoxic-ischemic injury both in vitro and in vivo. DARU, Journal of Pharmaceutical Sciences, 20 (1).

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Abstract

Background: Brain ischemia initiates several metabolic events leading to neuronal death. These events mediate large amount of damage that arises after some neurodegenerative disorders as well as transient brain ischemia. Melissa officinalis is considered as a helpful herbal plant in the prevention of various neurological diseases like Alzheimer that is related with oxidative stress. Methods. We examined the effect of Melissa officinalis on hypoxia induced neuronal death in a cortical neuronal culture system as in vitro model and transient hippocampal ischemia as in vivo model. Transient hippocampal ischemia was induced in male rats by tow vessel-occlusion for 20 min. After reperfusion, the histopathological changes and the levels inflammation, oxidative stress status, and caspase-3 activity in hippocampus were measured. Results: Cytotoxicity assays showed a significant protection of a 10 g/ml dose of Melissa against hypoxia in cultured neurons which was confirmed by a conventional staining (P<0.05). Melissa treatment decrease caspase3 activity (P<0.05) and TUNEL-positive cells significantly (P<0.01). Melissa oil has also inhibited malon dialdehyde level and attenuated decrease of Antioxidant Capacity in the hippocampus. Pro-inflammatory cytokines TNF-, IL-1 and HIF-1 mRNA levels were highly increased after ischemia and treatment with Melissa significantly suppressed HIF-1 gene expression (P<0.05). Discussion. Results showed that Melissa officinalis could be considered as a protective agent in various neurological diseases associated with ischemic brain injury. © 2012 Bayat et al.; licensee BioMed Central Ltd.

Item Type: Article
Additional Information: cited By 13
Uncontrolled Keywords: caspase 3; hypoxia inducible factor 1alpha; Melissa officinalis extract; messenger RNA; thiobarbituric acid reactive substance; tumor necrosis factor alpha, animal experiment; antioxidant activity; article; brain cortex; brain ischemia; brain nerve cell; cytotoxicity; DNA fragmentation; enzyme activity; gene expression; hippocampus; hypoxic ischemic encephalopathy; in vitro study; in vivo study; lipid peroxidation; male; Melissa officinalis; neuroprotection; nonhuman; oxidative stress; rat
Subjects: Neuroscience
Divisions: Faculty of Medicine > Basic Sciences > Department of Anatomy
Depositing User: editor . 2
Date Deposited: 01 Mar 2017 11:19
Last Modified: 12 Mar 2017 14:59
URI: http://eprints.kaums.ac.ir/id/eprint/739

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