Alpha-reductase 1 regulates spinal cord testosterone after morphine administration

Sharif, A. and Shoae-Hassani, A. and Sharif, S. and Banafshe, H.R. and Mortazavi-Tabatabaei, S.A. and Verdi, J. (2013) Alpha-reductase 1 regulates spinal cord testosterone after morphine administration. Neurological Sciences, 34 (1). pp. 19-23.

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The enzyme 5alpha-reductase 1 (5α-R1) that converts testosterone (T) to dihydrotestosterone (DHT) is present in many mammalian tissues including the spinal cord. It is established that morphine administration decreases spinal cord T levels, but the mechanism is still undetermined. Here, we investigated the link between T and the enzyme 5α-R1 in the spinal cord after morphine administration. For spinal cord steroid extraction, all the animals were killed 30 min, 2 h (acute) and 14 days (chronic) after first drug injection by decapitation. The whole spinal cord was removed and kept frozen at -20 C until T and DHT extraction. The effects of acute and chronic morphine administration on 5α-R1 expression in the adult male rat spinal cord were evaluated using RT-PCR. Spinal cord T and DHT levels were measured using radioimmunoassay before and after the morphine exposure. Morphine significantly reduced the T concentration after acute and chronic exposure in the spinal cord. In contrast, the 5α-R 1 expression and of course DHT levels increased the following chronic morphine administration. One important reason for the decreasing effect of morphine exposure on the spinal cord T level is due to an increase in the 5α-R1 levels. We suggest that morphine plays a regulatory role in metabolism of neurosteroids, especially T in the spinal cord via 5α-R1. © 2012 Springer-Verlag.

Item Type: Article
Additional Information: cited By 4
Uncontrolled Keywords: androstanolone; morphine; steroid 5alpha reductase 1; testosterone, adult animal; animal experiment; animal tissue; article; controlled study; drug exposure; male; morphine addiction; nonhuman; protein expression; radioimmunoassay; rat; regulatory mechanism; reverse transcription polymerase chain reaction; spinal cord, 3-Oxo-5-alpha-Steroid 4-Dehydrogenase; Animals; Dihydrotestosterone; Male; Morphine; Narcotics; Neurotransmitter Agents; Radioimmunoassay; Rats; Rats, Wistar; Real-Time Polymerase Chain Reaction; Spinal Cord; Testosterone
Subjects: Pharmacology, Toxicology and Pharmaceutics
Divisions: Faculty of Medicine > Basic Sciences > Department of Pharmacology
Depositing User: editor . 2
Date Deposited: 03 Mar 2017 12:35
Last Modified: 03 Mar 2017 12:35

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