Phylogenetic and biochemical characterization of a new halo-thermotolerant, biofilm-forming Bacillus from Saline Lake of Iran

Safary, A. and Moniri, R. and Mehdi Mirfiashemi, S. and Nikzad, H. and Akbarzadeh-Khiavi, M. (2013) Phylogenetic and biochemical characterization of a new halo-thermotolerant, biofilm-forming Bacillus from Saline Lake of Iran. Polish Journal of Microbiology, 62 (4). pp. 419-425.

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Abstract

In this study, five halotolerant Bacillus isolates from Aran-Bidgol Saline Lake in Iran were identified from saline environments. Screening of the bacteria led to the identification of a unique halo-thermotolerant Bacillus. On the basis of genetic and phenotypic data, this isolate was closely related to Bacillus licheniformis. But isolated Bacillus can be distinguished from B. licheniformis by salt tolerance, 16S rDNA sequence and some different physicochemical properties. Thus, suggested that the isolate was not the known Bacillus. Optical density analysis indicated strong biofilm formation for this strain. Also this isolate exhibited average tolerance to 1-25 mM concentrations of zinc and was sensitive to all concentrations of nickel. In biosurfactant production assay, this Bacillus exhibited the high activity for semi-quantitative oil displacement test (3.14 ± 0.02 cm2) and evaluated positive for drop-collapse test and hemolytic activity. Moreover, amylase, protease and DNase enzymes produced in presence of 10-20 salt of medium. Therefore, identified Bacillus could supply potential microbial materials for bioremediation purposes and biotechnological applications.

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: amylase; bacterial DNA; bacterial enzyme; biosurfactant; deoxyribonuclease; DNA 16S; proteinase; zinc, article; Bacillus; Bacillus licheniformis; Bacillus pumilus; Bacillus safensis; Bacillus sonorensis; bacterial strain; bacterium identification; bacterium isolate; biofilm; bioremediation; concentration response; controlled study; heat tolerance; hemolysis; Iran; lake; molecular phylogeny; nonhuman; nucleotide sequence; optical density; quantitative analysis; salinity; sequence homology, Bacillus; Biofilms; Gene Expression Regulation, Bacterial; Genotype; Iran; Lakes; Nickel; Phylogeny; RNA, Bacterial; RNA, Ribosomal, 16S; Salinity; Salt-Tolerance; Surface-Active Agents; Zinc
Subjects: Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Divisions: Faculty of Medicine > Basic Sciences > Department of Microbiology & Immunology
Depositing User: editor . 2
Date Deposited: 03 Mar 2017 13:03
Last Modified: 03 Mar 2017 13:03
URI: http://eprints.kaums.ac.ir/id/eprint/698

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