Dynamic changes of regulatory T cell and dendritic cell subsets in stable kidney transplant patients a prospective analysis

Nikoueinejad, H. and Amirzargar, A. and Sarrafnejad, A. and Einollahi, B. and Nafar, M. and Ahmadpour, P. and Pour-Reza-Gholi, F. and Sehat, O. and Lesanpezeshki, M. (2014) Dynamic changes of regulatory T cell and dendritic cell subsets in stable kidney transplant patients a prospective analysis. Iranian Journal of Kidney Diseases, 8 (2). pp. 130-138.

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Abstract

Introduction. We aimed to identify immune status of the stable kidney allografts from the point of some cellular changes that may occur after transplantation. Materials and Methods. This study considered 57 patients with no rejection during the 6 months after transplantation. Flow cytometric frequencies of circulatory CD4+CD25+FoxP3+ and CD8+CD28- regulatory T cells (Treg) as well as myeloid dendritic cells type 1 (MDC1) and type 2 (MDC2) and plasmacytoid dendritic cells (PDC) were measured before transplantation and 2 weeks and 1, 3, and 6 months after transplantation. Using adjusted model of repeated measure analysis, we assessed the influence of different parameters on different cell subsets. Results. The mean number of Tregs and PDCs decreased 2 weeks after transplantation and then increased as they reached their values before transplantation within a few months after transplantation. The mean MDC1s increased during 2 weeks and then decreased to its before-transplantation values within 6 months. The frequency of Tregs (r = 0.90) and MDC1s (r = 0.75) at month 3 could strongly predict their frequencies at month 6. Different variables including family relationship between donor and recipient, glomerular filtration rate, and human leukocyte antigen antibody mismatch did not change the frequency of different cell subsets during the time. Conclusions. The dynamism and circulatory changes in the frequency of Tregs and PDCs are opposite to MDCs after kidney transplantation. We describe these changes in a group of patients with stable graft; however, our study does not render any idea in patients with unstable or rejecting grafts.

Item Type: Article
Additional Information: cited By 4
Uncontrolled Keywords: CD45 antigen; creatinine; transcription factor FOXP3; alloantibody; biological marker; HLA antigen, adult; article; CD4+ CD25+ T lymphocyte; CD8+ T lymphocyte; cell differentiation; dendritic cell; family relation; female; flow cytometry; glomerulus filtration rate; graft recipient; human; immune status; immunosuppressive treatment; kidney transplantation; major clinical study; male; middle aged; myeloid dendritic cell; organ donor; plasmacytoid dendritic cell; prospective study; radioimmunoassay; regulatory T lymphocyte; blood; clinical trial; dendritic cell; histocompatibility; immunology; Iran; kidney transplantation; longitudinal study; metabolism; multicenter study; regulatory T lymphocyte; time; treatment outcome, Adult; Biological Markers; Dendritic Cells; Female; Flow Cytometry; Glomerular Filtration Rate; Histocompatibility; HLA Antigens; Humans; Iran; Isoantibodies; Kidney Transplantation; Longitudinal Studies; Male; Middle Aged; Prospective Studies; T-Lymphocytes, Regulatory; Time Factors; Treatment Outcome
Subjects: Biochemistry, Genetics and Molecular Biology
Divisions: Faculty of Medicine > Clinical Sciences > Department of Internal Medicine
Depositing User: editor . 2
Date Deposited: 04 Mar 2017 06:38
Last Modified: 04 Mar 2017 06:38
URI: http://eprints.kaums.ac.ir/id/eprint/552

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