Histological Survey of the Effect of Granulocyte-colony-stimulating Factor(G-CSF) on Bacterial Translocation and Wound Healing in Burned Mice

Moghadam, Somayeh. and Aghmiyuni, Zeinab. and Mohammad, Nazanin. and Anissian, Ali. and Azimi, Maryam. and Majidpour, Ali. and Nasirinezhad, Farinaz. and Roham, Maryam. (2019) Histological Survey of the Effect of Granulocyte-colony-stimulating Factor(G-CSF) on Bacterial Translocation and Wound Healing in Burned Mice. Archives of Trauma Research, 8 (3). pp. 149-154.

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Abstract

<b>Background:</b> Burn wound is an important cause of morbidity and mortality worldwide. Improving the host's immune system and removing the infection can be effective in healing wounds caused by burns. Granulocyte-colony-stimulating factor (G-CSF) stimulates both the bone marrow to produce granulocytes and the function of neutrophil precursors. The aim of this study was to examine the effect of G-CSF on removing infection and healing wound. <b>Materials and Methods:</b> A burn model was used to induce burns in 18 adult Balb/c mice, and their wounds were infected by <i>Acinetobacter baumannii</i> strains. Burned mice were divided into two groups (control and G-CSF) and treated daily by subcutaneous injections of normal saline (0.1 mL) and G-CSF (10 μg/kg). The wound healing process was evaluated by the morphological and histological assessments. <b>Results:</b> In morphological assay, the mean size of the wounds in the 3<sup>rd</sup> and 7<sup>th</sup> days of the treatment was significantly lower in the G-CSF treated group compared to the control group. Some of the histological parameters were evaluated, including the level of inflammation, re-epithelialization, angiogenesis, collagen deposition, the amount of granulation tissue, and fibroblast maturation. The results showed that inflammation was reduced in the G-CSF-treated group, and re-epithelialization and collagen deposition were increased insignificantly compared to the normal saline-treated group. Furthermore, bacterial translocation was reduced significantly in the G-CSF-treated group. <b>Conclusion:</b> G-CSF enhances wound closure and helps in wound healing by improving the immune system. It has also an anti-inflammatory role and reduces bacterial translocation.

Item Type: Article
Subjects: Medicine
Divisions: Archives of Trauma Research journal
Depositing User: editor . truma
Date Deposited: 23 Aug 2020 10:54
Last Modified: 23 Aug 2020 10:54
URI: http://eprints.kaums.ac.ir/id/eprint/5248

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