Detection of plasmid-mediated qnr genes among the quinolone-resistant Escherichia coli isolates in Iran

Firoozeh, F. and Zibaei, M. and Soleimani-Asl, Y. (2014) Detection of plasmid-mediated qnr genes among the quinolone-resistant Escherichia coli isolates in Iran. Journal of Infection in Developing Countries, 8 (7). pp. 818-822.

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Introduction: Plasmid-mediated quinolone resistance, which complicates treatment, has been increasingly identified in Escherichia coli isolates worldwide. The purpose of this study was to identify the plasmid-mediated qnrA and qnrB genes among the quinolone-resistant Escherichia coli isolated from urinary tract infections in Iran. Methodology: A total of 140 Escherichia coli isolates were collected between March and October 2012 from urinary tract infections in Khorram Abad, Iran. All isolates were tested for quinoloe resistance using the disk diffusion method. Also, all quinolone-resistant isolates were screened for the presence of the qnrA and qnrB genes by polymerase chain reaction. Minimum inhibitory concentrations (MICs) of ciprofloxacin for the qnr-positive isolates were determined. Results: One hundred sixteen (82.8) of 140 Escherichia coli isolates were nalidixic acid-resistant; among them, 14 (12.1) and 9 (7.8) were qnrA and qnrB-positive, respectively. Two quinolone-resistant isolates harbored both qnrA and qnrB. Among 63 ciprofloxacin-resistant isolates, 14 (22.2) and 9 (14.3) were found to carry qnrA and qnrB genes, respectively. The ciprofloxacin MIC range was 0.25-512 μg/mL for 23 qnr-positive Escherichia coli isolates, 18 of which had MICs values of 4-512 μg/mL. Conclusion: Our study shows that the frequency of plasmid-mediated quinolone resistance genes among E. coli isolates in Iran is high. © 2014 Firoozeh et al.

Item Type: Article
Additional Information: cited By 3
Uncontrolled Keywords: ciprofloxacin; levofloxacin; nalidixic acid; norfloxacin; ofloxacin; quinolone; antiinfective agent; ciprofloxacin; Escherichia coli protein; nalidixic acid; Qnr protein, E coli; quinolone derivative, antibiotic resistance; antibiotic sensitivity; article; bacterium isolation; broth dilution; controlled study; disk diffusion; Escherichia coli; gene; gene amplification; genetic screening; minimum inhibitory concentration; nonhuman; polymerase chain reaction; qnr gene A; qnr gene B; urinary tract infection; antibiotic resistance; drug effects; genetics; human; Iran; isolation and purification; microbial sensitivity test; microbiology; plasmid, Anti-Bacterial Agents; Ciprofloxacin; Drug Resistance, Bacterial; Escherichia coli; Escherichia coli Proteins; Humans; Iran; Microbial Sensitivity Tests; Nalidixic Acid; Plasmids; Quinolones; Urinary Tract Infections
Subjects: Immunology and Microbiology
Divisions: Faculty of Medicine > Basic Sciences > Department of Microbiology & Immunology
Depositing User: editor . 2
Date Deposited: 04 Mar 2017 07:17
Last Modified: 04 Mar 2017 07:17

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