Molecular identification and prevalence of Malassezia species in pityriasis versicolor patients from Kashan, Iran

Talaee, R. and Katiraee, F. and Ghaderi, M. and Erami, M. and Alavi, A.K. and Nazeri, M. (2014) Molecular identification and prevalence of Malassezia species in pityriasis versicolor patients from Kashan, Iran. Jundishapur Journal of Microbiology, 7 (8).

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Background: Malassezia species are lipophilic yeasts found on the skin surface of humans and other warm-blooded vertebrates. It is associated with various human diseases, especially pityriasis versicolor, which is a chronic superficial skin disorder. Objectives: The aim of the present study was to identify Malassezia species isolated from patients' samples affected by pityriasis versicolor, using molecular methods in Kashan, Iran. Patients and Methods: A total of 140 subjects, suspected of having pityriasis versicolor from Kashan, were clinically diagnosed and then confirmed by direct microscopic examination. The scraped skin specimens were inoculated in modified Dixon's medium. DNA was extracted from the colonies and PCR amplification was carried out for the 26s rDNA region. PCR products were used to further restriction fragment length polymorphism by CfoI enzyme. Results: Direct examination was positive in 93.3 of suspected pityriasis versicolor lesions. No statistically significant difference was observed in the frequency of Malassezia species between women and men. The highest prevalence of tinea versicolor was seen in patients 21-30 years-of-age. No difference could be seen in the frequency of Malassezia species depending on the age of the patients. In total, 65 of patients with pityriasis versicolor had hyperhidrosis. The most commonly isolated Malassezia species in the pityriasis versicolor lesions were; Malassezia globosa (66), M. furfur (26), M. restricta (3), M. sympodialis (3), and M. slooffiae (2). Malassezia species were mainly isolated from the neck and chest. Conclusions: This study showed M. globosa to be the most common Malassezia species isolated from Malassezia skin disorders in Kashan, Iran. The PCR-RFLP method was useful in the rapid identification of the Malassezia species. By using these methods, the detection and identification of individual Malassezia species from clinical samples was substantially easier. © 2014, Ahvaz Jundishapur University of Medical Sciences; Published by Kowsar Corp.

Item Type: Article
Additional Information: cited By 4
Uncontrolled Keywords: DNA 26S; enzyme CfoI; fungal enzyme; unclassified drug, adult; aged; article; child; controlled study; DNA extraction; female; fungal colonization; fungus culture; fungus identification; fungus isolation; groups by age; human; human tissue; hyperhidrosis; hyperpigmentation; hypopigmentation; Iran; major clinical study; Malassezia; Malassezia furfur; Malassezia globosa; Malassezia restricta; Malassezia slooffiae; Malassezia sympodialis; male; microscopy; middle aged; modified Dixon medium; molecular diagnosis; molecular epidemiology; molecular model; nonhuman; preschool child; prevalence; restriction fragment length polymorphism; school child; sex difference; skin pigmentation; tinea versicolor; tissue and organ preparation and culture; very elderly; young adult
Subjects: Parasitology
Divisions: Faculty of Medicine > Basic Sciences > Department of Parasitological & Mycology
Depositing User: editor . 2
Date Deposited: 05 Mar 2017 08:03
Last Modified: 05 Mar 2017 08:03

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