Molecular and serological techniques to determine the acute and chronic phase of toxoplasmosis in HIV patients

Shokri, F. and Abbaszadeh, R. and Rostamnezhad, M. and Heidari Digesara, S. and Marandi, N. and Bairami Kuzehkanan, A. and Abolhassani, M. and Gharavi, M.J. (2019) Molecular and serological techniques to determine the acute and chronic phase of toxoplasmosis in HIV patients. Journal of Babol University of Medical Sciences, 21 (1). pp. 140-146.

Full text not available from this repository.
Official URL:


BACKGROUND AND OBJECTIVE: Toxoplasma gondii is associated with several complications including neurological problems, ocular damage and encephalitis in immunodeficiency individuals. Early diagnosis of this infection can lead to better management of this disease. Therefore, this study was conducted to determine the presence of Toxoplasma gondii with two serologic and molecular methods in HIV-infected individuals. METHODS: In this cross-sectional study, 102 male patients with HIV with a mean age of 40 ± 9.2 years were examined. The serum sample was used for ELISA to determine the acute and chronic phase and cellular samples using Real Time-PCR for determining the acute phase of the disease. The relationship between age groups and the HIV transmission pathway, as well as the age group, was compared with the results of the Toxoplasma gondii test. FINDINGS: Out of 102 samples tested for IgM anti-Toxoplasma gondii antigen by ELFA, all (100) samples were negative, but for anti-IgG anti-parasite, 44 samples (43.1) were positive and 58 Sample (56.9) was negative. Out of 102 samples tested by RT-PCR, all (100) samples were negative for Toxoplasma DNA. There was a statistically significant relationship between age groups and transmission pathways (p<0.001), as well as between age groups with anti-Toxoplasma gondii IgG levels (p<0.001). CONCLUSION: According to the results of this study, the use of IgM-ELFA and PCR-RT methods for the diagnosis of acute phase and IgG-ELFA in the chronic phase of the disease is important. With the diagnosis of chronic form of toxoplasmosis, preventive treatments can be used in HIV+ patients. © 2019, Babol University of Medical Sciences. All rights reserved.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: immunoglobulin G; immunoglobulin M; repetitive DNA, adult; Article; blood sampling; cross-sectional study; diagnostic test accuracy study; disease transmission; DNA extraction; enzyme linked fluourescent assay; enzyme linked immunosorbent assay; fluorescence analysis; human; Human immunodeficiency virus infected patient; Human immunodeficiency virus infection; major clinical study; male; middle aged; molecular biology; parasite transmission; prevalence; real time polymerase chain reaction; serology; Toxoplasma gondii; toxoplasmosis
Subjects: Medicine
Immunology and Microbiology
Pharmacology, Toxicology and Pharmaceutics
Divisions: Faculty of Medicine > Basic Sciences > Department of Microbiology & Immunology
Depositing User: ART . editor
Date Deposited: 29 Dec 2019 10:25
Last Modified: 29 Dec 2019 10:25

Actions (login required)

View Item View Item