Anticonvulsant effects of thiamine on pentylenetetrazole-induced seizure in mice

Mesdaghinia, A. and Alinejad, M. and Abed, A. and Heydari, A. and Banafshe, H.R. (2019) Anticonvulsant effects of thiamine on pentylenetetrazole-induced seizure in mice. Nutritional Neuroscience, 22 (3). pp. 165-173.

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Objectives: Thiamine serves as a cofactor for several enzymes involved in brain function and neurotransmitters biosynthesis. Thiamine-dependent enzymes are important for oxidant stress defenses. Several studies have reported that thiamine deficiency in the central nervous system reduces seizure threshold. The present study was designed to investigate the effect of acute and chronic administration of thiamine alone and in combination with sub-effective dose of diazepam on pentylenetetrazole (PTZ)-induced tonic�clonic seizures in mice. Methods: Animals were randomly divided into control and experimental groups. In experimental groups, thiamine (50, 100, and 200 mg/kg i.p.) was administered acutely or chronically (once a day, for 14 days). Slow intravenous infusion of PTZ (5 mg/ml) by infusion pump with a constant rate (0.3 ml/min) was used to induce clonic and tonic seizures. Results: Acute injection of thiamine (50, 100, and 200 mg/kg i.p.) did not increase seizure threshold significantly, but chronic treatment with thiamine (200 mg/kg i.p.) increases the clonic and tonic seizure threshold. Moreover, the combination of sub-effective dose of thiamine (100 mg/kg) and diazepam (0.1 mg/kg) significantly increased seizure threshold and enhanced the anticonvulsant effect of diazepam at ineffective dose (0.1 mg/kg). Discussion: Our results suggest that thiamine can be considered as a potential add-on treatment in deficient and non-deficient thiamine epileptic patients. Co-administration of this vitamin with classic antiepileptics to decrease the required doses of regular drugs may be recommended. Nevertheless, more well-designed studies may be executed to provide further accurate information. © 2017, © 2017 Informa UK Limited, trading as Taylor & Francis Group.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: diazepam; thiamine; anticonvulsive agent; diazepam; pentetrazole; thiamine, animal experiment; animal model; anticonvulsant activity; Article; clinical assessment; controlled study; male; mouse; nonhuman; oxidative stress; pentylenetetrazole-induced seizure; priority journal; seizure threshold; tonic clonic seizure; animal; Bagg albino mouse; chemically induced; disease model; dose response; epilepsy; seizure, Animals; Anticonvulsants; Diazepam; Disease Models, Animal; Dose-Response Relationship, Drug; Epilepsy; Male; Mice, Inbred BALB C; Pentylenetetrazole; Seizures; Thiamine
Subjects: Physiology
Pharmacology, Toxicology and Pharmaceutics
Divisions: Faculty of Medicine > Basic Sciences > Department of Pharmacology
Depositing User: ART . editor
Date Deposited: 30 Dec 2019 10:38
Last Modified: 30 Dec 2019 10:38

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