Therapeutic effect of Hedera helix alcoholic extract against Cutaneous Leishmaniasis caused by Leishmania major in Balb/C mice

Hooshyar, H. and Talari, S.A. and Feyzi, F. (2014) Therapeutic effect of Hedera helix alcoholic extract against Cutaneous Leishmaniasis caused by Leishmania major in Balb/C mice. Jundishapur Journal of Microbiology, 7 (4).

Full text not available from this repository.
Official URL:


Background: Cutaneous leishmaniasis (CL) is common and endemic in many areas of Iran, caused by species of a protozoan parasite belonging to the genus Leishmania. There is not any effective vaccine against leishmaniasis; so, therapy is important for prevention and separation of disease. Herbal extract for treatment of CL is cost-effective, applicable topically to lesions, and can avoid the development of drug resistance. Objectives: The aim of this study was to evaluate the in vivo activity of an alcoholic extract of Hedera helix (a native Iranian plant) on the experimental ulcer of zoonotic CL in Balb/c mice. Materials and Methods: At least 5x l06 promastigotes of Leishmania major (MHOM/64/IR/ER75) were inoculated subcutaneously in the tail base of Balb/c mice. Fifty six infected mice were distributed in four groups, two groups (16 mice for 20 alcoholic extract of H. helix and 13 for 70 extract) were used as experimental groups, one (15 mice) as placebo control (Control A), and one (12 mice) as negative control. Treatment effects of two concentrations were determined by comparison of placebo and nontreated groups via measuring the size of skin lesions and the number of parasitologically positive and negative mice after the therapy period. Results: This study showed that the main lesion size did not decrease significantly, or the small lesions did not completely disappear after treatment by H. helix alcoholic extract. Amastigotes counts (mean ± SD) of the skin lesions decreased in control A and 20 concentration groups, but in negative control and 70 concentration groups the number of parasites did not reduce. Conclusions: The present study did not support the in vivo antileishmanial effect of H. helix extract. We recommend further studies using major components of H. helix, especially hederasaponin (saponin K10), to investigate the antileishmanial effect of this plant on L. major. © 2014, Ahvaz Jundishapur University of Medical Sciences; Published by Kowsar Corp.

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: alcohol; hedelix; placebo, amastigote; animal experiment; animal model; article; concentration response; controlled study; drug effect; in vivo study; Leishmania major; mouse; nonhuman; parasitology; promastigote; skin defect; skin leishmaniasis
Subjects: Parasitology
Divisions: Faculty of Medicine > Basic Sciences > Department of Parasitological & Mycology
Depositing User: editor . 2
Date Deposited: 05 Mar 2017 21:23
Last Modified: 07 Jun 2017 14:05

Actions (login required)

View Item View Item