Intranasal administration of endometrial mesenchymal stem cells as a suitable approach for Parkinson�s disease therapy

Bagheri-Mohammadi, S. and Alani, B. and Karimian, M. and Moradian-Tehrani, R. and Noureddini, M. (2019) Intranasal administration of endometrial mesenchymal stem cells as a suitable approach for Parkinson�s disease therapy. Molecular Biology Reports, 46 (4). pp. 4293-4302.

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Abstract

This study aimed to investigate the therapeutic effects of intranasal administration of human endometrium-derived stem cells (HEDSCs) in the mouse model of Parkinson�s disease (PD). Thirty days after intrastriatal injection of 6-OHDA, HEDSCs were administrated intranasally in three doses (104, 5 � 104 and 105 cells µl�1). During 120 days after stem cell administration, behavioral tests were examined. Then the mice were sacrificed and the fresh section of the substantia nigra pars compacta (SNpc) was used for detection of HEDSCs-GFP labeled by fluorescence microscopy method. In addition, immunohistochemistry was used to assay GFP, human neural Nestin, and tyrosine hydroxylase (TH) markers in the fixed brain tissue at the SNpc. Our data revealed that behavioral parameters were significantly improved after cell therapy. Fluorescence microscopy assay in fresh tissue and GFP analysis in fixed tissue were showed that the HEDSCs-GFP labeled migrated to SNpc. The data from immunohistochemistry revealed that the Nestin as a differential neuronal biomarker was expressed in SNpc. Also, TH as a dopaminergic neuron marker significantly increased after HEDSCs therapy in an optimized dose 5 � 104 cells µl�1. Our results suggest that intranasal administration of HEDSCs improve the PD symptoms in the mouse model of PD dose-dependent manner as a noninvasive method. © 2019, Springer Nature B.V.

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: biological marker; green fluorescent protein; nestin; oxidopamine; tyrosine 3 monooxygenase, akinesia; animal behavior; animal experiment; animal model; animal tissue; Article; catalepsy; cell migration; circling behavior; clinical effectiveness; controlled study; dopaminergic nerve cell; endometrial mesenchymal stem cell transplantation; fluorescence microscopy; human; human cell; immunohistochemistry; intranasal drug administration; male; mesenchymal stem cell transplantation; mouse; mouse model; non invasive procedure; nonhuman; Parkinson disease; protein expression; rotarod test; substantia nigra pars compacta
Subjects: Physiology
Pathology
Divisions: Faculty of Medicine > Basic Sciences > Applied Cell Sciences
Depositing User: ART . editor
Date Deposited: 31 Dec 2019 15:16
Last Modified: 31 Dec 2019 15:16
URI: http://eprints.kaums.ac.ir/id/eprint/4477

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