Molecular detection of antibiotic resistance genes, and class I, and II integrons in Salmonella enteritidis isolated from Iranian one-day-old chicks

Ghazalibina, M. and Farahani, R.K. and Mansouri, S. and Meskini, M. and Farahani, A.H.K. and Khaledi, A. (2019) Molecular detection of antibiotic resistance genes, and class I, and II integrons in Salmonella enteritidis isolated from Iranian one-day-old chicks. Gene Reports, 16.

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DOI: UNSPECIFIED

Abstract

This study aimed to investigate the molecular detection of antibiotic resistance genes, and class 1, and II integrons in Salmonella enteritidis isolated from Iranian one-day-old chicks. In this cross-sectional study, 650 samples were collected from one-day-old chicks from different regions of Iran and transferred to the Central Veterinary Laboratory-Iran Veterinary Organization (CVL-IVO). The prevalence of Integrons and resistance genes were determined by PCR. The pattern of antibiotic resistance was determined using Kirby-Bauer disk diffusion method. The data were analyzed using SPSS software through consensus table, and Chi-square test. Results showed the prevalence 33.3 of integrons. Frequency class I and II integrons were 26.6 and 6.6, respectively. All isolates had GES, and gyrB genes, but 6.6 of those have DHAM gene. Other isolates did not show any resistance gene. The highest resistance was observed to Nalidixic acid, Penicillin and Nitrofurantoin (100). The lowest resistance was reported to Trimethoprim with prevalence 6.6. Our results showed a high prevalence of class � integrons, and a correlation between presence of class I and II integrons with resistance to Nalidixic acid, Penicillin, Nitrofurantoin, Colistin, Levofloxacin, and Ciprofloxacin in Salmonella enteritidis. © 2019

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: bacterial DNA; bacterial RNA; ciprofloxacin; colistin; levofloxacin; nalidixic acid; nitrofurantoin; penicillin derivative; trimethoprim, antibiotic resistance; antibiotic sensitivity; Article; bacterium isolation; chi square test; chick; cross-sectional study; disk diffusion; DNA extraction; food contamination; integron; Iran; molecular genetics; nonhuman; nucleotide sequence; priority journal; real time polymerase chain reaction; RNA extraction; Salmonella enterica serovar Enteritidis
Subjects: Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Divisions: Faculty of Medicine > Basic Sciences > Department of Microbiology & Immunology
Depositing User: ART . editor
Date Deposited: 31 Dec 2019 15:35
Last Modified: 31 Dec 2019 15:35
URI: http://eprints.kaums.ac.ir/id/eprint/4465

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