The protective effect of bone marrow mesenchymal stem cells in a rat model of ischemic stroke via reducing the C-Jun N-terminal kinase expression

Vahidinia, Z. and Azami Tameh, A. and Nejati, M. and Beyer, C. and Talaei, S.A. and Etehadi Moghadam, S. and Atlasi, M.A. (2019) The protective effect of bone marrow mesenchymal stem cells in a rat model of ischemic stroke via reducing the C-Jun N-terminal kinase expression. Pathology Research and Practice, 215 (9).

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Abstract

Ischemic stroke is the main cause of disability and mortality worldwide. Apoptosis and inflammation have an important role in ischemic brain injury. Mesenchymal stem cells (MSCs) have protective effects on stroke treatment due to anti-inflammatory properties. The inhibition of the C-Jun N-terminal kinase (JNK) pathway may be one of the molecular mechanisms of the neuroprotective effect of MSCs in ischemic brain injury. Twenty-eight male Wistar rats were divided randomly into 3 groups. Except the sham group, others subjected to transient middle cerebral artery occlusion (tMCAO). Bone marrow MSCs or saline were injected 3 h after tMCAO. Sensorimotor behavioral tests were performed 24 and 72 h after ischemia and reperfusion (I/R). The rats were sacrificed 72 h after I/R and infarct volume was measured by TTC staining. The number of apoptotic neurons and astrocytes in the peri-infarct area was assessed by TUNEL assay. The morphology of cells was checked by Nissl staining, and the expression of p-JNK was detected by immunohistochemistry and Western blot. Behavioral scores were improved and infarct volume was reduced by MSCs 24 h and 72 h after tMCAO. TUNEL assay showed that neuronal apoptosis and astroglial activity in the penumbra region were reduced by MSCs. Also, Nissl staining showed lower neuronal apoptosis in BMSCs-treated rats compared to controls. JNK phosphorylation which was profoundly induced by ischemia was significantly decreased after MSCs treatment. We concluded that anti-apoptotic and anti-inflammatory effects of MSCs therapy after brain ischemia may be associated with the down-regulation of p-JNK. © 2019 Elsevier GmbH

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: stress activated protein kinase; tissue plasminogen activator, adult; animal cell; animal experiment; animal model; animal tissue; Article; astrocyte; bone marrow derived mesenchymal stem cell; brain ischemia; cerebral ischemia reperfusion injury; controlled study; enzyme inhibition; experimental behavioral test; immunohistochemistry; infarct volume; male; mesenchymal stem cell transplantation; middle cerebral artery occlusion; neuroapoptosis; neuroprotection; Nissl staining; nonhuman; rat; rat model; TUNEL assay; Western blotting; Wistar rat
Subjects: Anatomy Morphology
Pathology
Divisions: Faculty of Medicine > Basic Sciences > Department of Anatomy
Depositing User: ART . editor
Date Deposited: 01 Jan 2020 11:06
Last Modified: 01 Jan 2020 11:06
URI: http://eprints.kaums.ac.ir/id/eprint/4460

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