Evaluation of ebselen supplementation on cryopreservation medium in human semen

Naeini, Z.K. and Hassani, H. and Nikzad, H. (2014) Evaluation of ebselen supplementation on cryopreservation medium in human semen. Iranian Journal of Reproductive Medicine, 12 (4). pp. 249-256.

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DOI: UNSPECIFIED

Abstract

Background: An effect of cryopreservation on human sperm is sublethal cryodamage, in which cell viability post-thaw is lost more rapidly at later times than in fresh cells. Objective: This study examined whether the addition of an antioxidant to cryopreservation medium could improve the post-thaw parameters and evaluation of sperm chromatin quality of cryopreserved human spermatozoa from men with normal semen parameters. Materials and Methods: Semen samples (n=35) were collected by masturbation and assessed following WHO standards. Individual samples were classified as two portions. One portion (n=10) was for elucidate the concentration of ebselen.Then the samples(n=25) were divided in to 5groups.The first aliquot remained fresh.The second aliquots was mixed with cryopreservation medium.The third aliquots were mixed with cryopreservation medium containing solvent of ebselen.The forth and fifth aliquots were mixed with cryopreservation medium containing 1.25 and 2.5 μm of ebselen.Samples were frozen and thawed samples were assessed for sperm parameters.Three-way ANOVA Multivariate measures were used to assess. According to this assesment the differences are observed in existent groups in post-thaw count, motility index, vitality staining, and morphology and DNA fragmentation. Results: After freezing the media containing of ebselen, DNA fragmentation is significantly different in comparison with control group. ebselen with 1.25 μm dose was significantly associated with post-thaw DNA fragmentation (p=0.047). Similarly ebselen with 2.5 μm dose was significantly associated with post-thaw DNA fragmentation (p=0.038). But other parameters were not altered. Conclusion: These results suggest that the addition of ebselen to cryopreservation medium doesnot improve post-thaw parameters and DNA fragmentation of sperm.

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: ebselen, article; cell structure; cell viability; chromatin; concentration (parameters); controlled study; cryopreservation; culture medium; DNA fragmentation; freeze thawing; human; male; semen analysis; sperm preservation; spermatozoon count; spermatozoon motility
Subjects: Anatomy Morphology
Biochemistry, Genetics and Molecular Biology
Divisions: Faculty of Medicine > Basic Sciences > Department of Anatomy
Depositing User: editor . 2
Date Deposited: 05 Mar 2017 22:18
Last Modified: 05 Mar 2017 22:18
URI: http://eprints.kaums.ac.ir/id/eprint/443

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