Association of EGFR gene mutations exons 18�21 with glioblastoma multiform cancer: A descriptive and cross-sectional study

Matini, A.H. and Tayebi, M.S. and Rezvani, Z. and Vakili, Z. and Haddad Kashani, H. (2019) Association of EGFR gene mutations exons 18�21 with glioblastoma multiform cancer: A descriptive and cross-sectional study. Gene Reports, 17.

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DOI: UNSPECIFIED

Abstract

Background: Glioblastoma multiforme (GBM), as the most aggressive type of glioma, is grouped as grade IV according to WHO classification. Methods: We included in the present study 20 Iranian patients with GMB, diagnosed between 2013 and 2018. DNA extraction was performed on 10 μm sections from paraffin blocks containing sample tissue. The extracted DNAs were used for amplification by PCR method. PCR products were then subjected to hybridization in order to detect mutations of EGFR. Results: In total, 20 of 20 (100) GBM samples studied had at least one mutation of EGFR. Mutation of G719A in 9 cases, mutations of G719C, G719S and T790M in 16 cases, mutation of E746T751delinesVA in 19 cases, mutations of E746S752delines in 2 cases and mutation of L861Q in 13 cases were observed. However, no significant relationship between EGFR mutations and sex, age, EGFR expression, necrosis, vascular proliferation and atypia was identified. Conclusions: EGFR mutation was found in 100 of patients with GBM. This finding may open avenues for future investigations to explore the effect of combining targeted EGFR therapy with conventional therapies in GMB management. © 2019

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: epidermal growth factor receptor; tumor marker, adult; Article; clinical article; controlled study; cross-sectional study; DNA extraction; DNA hybridization; exon; female; gene amplification; gene expression; gene mutation; genetic association; glioblastoma; human; human tissue; Iranian people; male; polymerase chain reaction; priority journal
Subjects: Anatomy Morphology
Pathology
Biochemistry, Genetics and Molecular Biology
Divisions: Faculty of Medicine > Basic Sciences > Department of Pathology& Histology
Depositing User: ART . editor
Date Deposited: 05 Jan 2020 11:21
Last Modified: 05 Jan 2020 11:21
URI: http://eprints.kaums.ac.ir/id/eprint/4381

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