Improved osteogenic differentiation of human induced pluripotent stem cells cultured on polyvinylidene fluoride/collagen/platelet-rich plasma composite nanofibers

Abazari, M.F. and Soleimanifar, F. and Amini Faskhodi, M. and Mansour, R.N. and Amini Mahabadi, J. and Sadeghi, S. and Hassannia, H. and Saburi, E. and Enderami, S.E. and Khani, M.M. and Zare Karizi, S. (2020) Improved osteogenic differentiation of human induced pluripotent stem cells cultured on polyvinylidene fluoride/collagen/platelet-rich plasma composite nanofibers. Journal of Cellular Physiology, 235 (2). pp. 1155-1164.

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Blood transfusion or blood products, such as plasma, have a long history in improving health, but today, platelet-rich plasma (PRP) is used in various medical areas such as surgery, orthopedics, and rheumatology in many ways. Considering the high efficiency of tissue engineering in repairing bone defects, in this study, we investigated the combined effect of nanofibrous scaffolds in combination with PRP on the osteogenic differentiation potential of human induced pluripotent stem cells (iPSCs). Electrospinning was used for fabricating nanofibrous scaffolds by polyvinylidene fluoride/collagen (PVDF/col) with and without PRP. After scaffold characterization, the osteoinductivity of the fabricated scaffolds was studied by culturing human iPSCs under osteogenic medium. The results showed that PRP has a considerable positive effect on the biocompatibility of the PVDF/col nanofibrous scaffold when examined by protein adsorption, cell attachment, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. In addition, the results obtained from alkaline phosphatase activity and calcium content assays demonstrated that nanofibers have higher osteoinductivity while grown on PRP-incorporated PVDF/col nanofibers. These results were also confirmed while the osteogenic differentiation of the iPSCs was more investigated by evaluating the most important bone-related genes expression level. According to the results, it can be concluded that PVDF/col/PRP has much more osteoinductivity while compared with the PVDF/col, and it can be introduced as a promising bone bio-implant for use in bone tissue engineering applications. © 2019 Wiley Periodicals, Inc.

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: alkaline phosphatase; beta 2 microglobulin; collagen; molecular scaffold; nanofiber; osteocalcin; osteonectin; polyvinylidene fluoride; transcription factor RUNX2, adsorption; Article; bone development; cell adhesion; cell differentiation; electrospinning; female; gene expression; human; human cell; induced pluripotent stem cell; male; normal human; osteoblast; osteoclastogenesis; priority journal; scanning electron microscopy; surface property; tensile strength; thrombocyte rich plasma; tissue engineering
Subjects: Pathology
Biochemistry, Genetics and Molecular Biology
Divisions: Faculty of Medicine > Basic Sciences > Applied Cell Sciences
Depositing User: ART . editor
Date Deposited: 05 Jan 2020 13:00
Last Modified: 05 Jan 2020 13:00

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