Tavakol, S. and Zare, S. and Hoveizi, E. and Tavakol, B. and Rezayat, S.M. (2019) The impact of the particle size of curcumin nanocarriers and the ethanol on beta1-integrin overexpression in fibroblasts: A regenerative pharmaceutical approach in skin repair and anti-aging formulations. DARU, Journal of Pharmaceutical Sciences.
Full text not available from this repository.Abstract
Background: Since women pay more attention to their skin�s health, pharmaceutical companies invest heavily on skin care product development. Further, the success of drug nano-carriers in passing through the skin justifies the need to conduct studies at the nano-scale. β1-integrin down regulation has been proposed as a sign of skin aging. Methods: Six drug nano-carriers (50 and 75 nm) were prepared at three ethanol concentrations (0, 3,and 5) and different temperatures. Then, the impact of Nanocarriers on fibroblasts were investigated. Results: DLS showed that increasing ethanol concentration decreased the surface tension that caused a decrease in the particle size in non-temperature formulations while increasing the temperature to 60 °C to lower Gibbs free energy increased the particle size. Ethanol addition decreased β1-integrin over-expression, whereas larger nano-carriers induced an over-expression of β1-integrin, Bcl2/Bax ratio, and an increase in live cell number. β1-integrin over-expression did not correlate with the rate of fibroblast proliferation and NFκB expression. An increase in fibroblast mortality in relation to smaller nano-carriers was not only due to the increase in Bax ratio, but was related to NFκB over-expression. Conclusion: The development of a regenerative pharmaceutical approach in skin repair was based on the effect of particle size and ethanol concentration of the drug nano-carriers on the expression of β1-integrin in fibroblasts. A curcumin nanoformulation sized 77 nm and containing of 3 ethanol was more effective in increasing β1-integrin gene over-expression, anti-apoptosis of fibroblast cells (Bcl2/Bax ratio), and in decreasing Bax and NFκB gene expression than that with a particle size of 50 nm. Such a formulation may be considered a valuable candidate in anti-aging and wound-healing formulations. Figure not available: see fulltext.. © 2019, Springer Nature Switzerland AG.
| Item Type: | Article |
|---|---|
| Additional Information: | cited By 0 |
| Subjects: | Biochemistry, Genetics and Molecular Biology Pharmacology, Toxicology and Pharmaceutics |
| Divisions: | Faculty of Medicine > Basic Sciences > Department of Pharmacology |
| Depositing User: | ART . editor |
| Date Deposited: | 22 Jun 2019 10:40 |
| Last Modified: | 22 Jun 2019 10:40 |
| URI: | http://eprints.kaums.ac.ir/id/eprint/4305 |
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