Molecular characterization of class 1, 2 and 3 integrons in clinical multi-drug resistant Klebsiella pneumoniae isolates

Firoozeh, F. and Mahluji, Z. and Khorshidi, A. and Zibaei, M. (2019) Molecular characterization of class 1, 2 and 3 integrons in clinical multi-drug resistant Klebsiella pneumoniae isolates. Antimicrobial Resistance and Infection Control, 8 (1).

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Abstract

Background: The aim of this study was to characterize class 1,2 and 3 integrons in clinical MDR Klebsiella pneumoniae isolates in Kashan, Iran. Methods: One hundred-eighty one Klebsiella pneumoniae were recovered from clinical specimens during November 2013 to October 2014. Antimicrobial susceptibility patterns were determined by disk diffusion method according to the Clinical and Laboratory Standards Institute (CLSI) guidelines for detection of MDR strains. Of the 181 Klebsiella pneumoniae, 146 (80.7) of isolates were isolated from nosocomial infected patients and 150 (82.9) identified as MDR isolates. The PCR amplification was used to show presence of class 1, 2 and 3 integrons among MDR strains. The PCR method and sequencing were used for evaluation of cassette content of integrons. Results: Of the MDR K. pneumoniae isolates, 150 (100) and 55 (36.7) carried intI1 and intI2 genes, respectively. None of the MDR Klebsiella pneumoniae isolates carried class 3 integrons. Amplification of conserved segment (CS) of class 1 and class 2 integrons revealed 10 different arrays including: No. cassette; dfrA5, dfrA30; aadA2; aadA2, dfrA12; dfrA17, aadA5, aadA4; dfrA5, dfrA30, aadA2; dfrA5, dfrA30, aadA2, dfrA12, dfrA5, dfrA30, dfrA17, aadA5, aadA4; aadA2, aadA2, dfrA12; dfrA5, dfrA30, aadA2, aadA2, dfrA12 and 4 arrays including: No. cassette; aadA1; dfrA1-sat1; aadA1, dfrA1-sat1, respectively. Conclusions: The finding of present study revealed a high prevalence of integrons especially class 1 among MDR K. pneumoniae isolates from nosocomial infections in Kashan, which led to rapid extension of MDR strains. © 2019 The Author(s).

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: antibiotic resistance; antibiotic sensitivity; Article; bacterial strain; bacterium identification; bacterium isolate; clinical protocol; controlled study; disk diffusion; DNA extraction; electrophoresis; gene amplification; gene cassette; gene sequence; integron; integron class 1; integron class 2; integron class 3; Iran; Klebsiella pneumoniae; microarray analysis; molecular dynamics; nonhuman; polymerase chain reaction; priority journal
Subjects: Immunology and Microbiology
Divisions: Faculty of Medicine > Basic Sciences > Department of Microbiology & Immunology
Depositing User: ART . editor
Date Deposited: 24 Aug 2019 10:51
Last Modified: 24 Aug 2019 10:51
URI: http://eprints.kaums.ac.ir/id/eprint/4192

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