N-acetylcysteine compared to metformin, improves the expression profile of growth differentiation factor-9 and receptor tyrosine kinase c-kit in the oocytes of patients with polycystic ovarian syndrome

Cheraghi, E. and Soleimani Mehranjani, M. and Shariatzadeh, S.M.A. and Nasr Esfahani, M.H. and Alani, B. (2018) N-acetylcysteine compared to metformin, improves the expression profile of growth differentiation factor-9 and receptor tyrosine kinase c-kit in the oocytes of patients with polycystic ovarian syndrome. International Journal of Fertility and Sterility, 11 (4). pp. 270-278.

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Abstract

Background: Paracrine disruption of growth factors in women with polycystic ovarian syndrome (PCOS) results in production of low quality oocyte, especially following ovulation induction. The aim of this study was to investigate the effects of metformin (MET), N-acetylcysteine (NAC) and their combination on the hormonal levels and expression profile of GDF-9, BMP-15 and c-kit, as hallmarks of oocyte quality, in PCOS patients. Materials and Methods: This prospective randomized, double-blind, placebo controlled trial aims to study the effects of MET, NAC and their combination (MET+NAC) on expression of GDF-9, BMP-15 and c-kit mRNA in oocytes 10 at the germinal vesicle (GV) stage, 10 at the MI stage, and 10 at the MII stage from per group derived following ovulation induction in PCOS. Treatment was carried out for six weeks, starting on the third day of previous cycle until oocyte aspiration. The expression of GDF9, BMP15 and c-kit were determined by quantitative real time polymerase chain reaction (RT-qPCR) and western blot analysis. Data were analyzed with one-way ANOVA. Results: The follicular fluid (FF) level of c-kit protein significantly decreased in the NAC group compared to the other groups. Significant correlations were observed between the FF soluble c-kit protein with FF volume, androstenedione and estradiol. The GDF-9 expression in unfertilized mature oocytes were significantly higher in the NAC group compared to the other groups (P<0.001). Similar difference was not observed between the MET, NAC+MET and control groups. The c-kit expression in unfertilized mature oocytes were significantly lower in the NAC group compared to the other groups (P<0.001). Similar difference was not observed between the MET, NAC+MET and control groups (Registration number: IRCT201204159476N1). Conclusion: We concluded that NAC can improve the quality of oocytes in PCOS. © 2017, Royan Institute (ACECR). All rights reserved.

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: acetylcysteine; androstenedione; bone morphogenetic protein 15; buserelin acetate; chorionic gonadotropin; estradiol; follitropin; growth differentiation factor 9; luteinizing hormone; metformin; progesterone; protein tyrosine kinase; recombinant follitropin; stem cell factor receptor; testosterone, abdominal discomfort; adult; Article; body mass; comparative study; controlled study; diarrhea; double blind procedure; down regulation; enzyme linked immunosorbent assay; female; follicular aspiration; gene expression; human; major clinical study; menstrual cycle; nausea; oocyte; oocyte retrieval; ovary follicle fluid; ovary polycystic disease; ovulation induction; prospective study; randomized controlled trial; real time polymerase chain reaction; urogenital system parameters; Western blotting
Subjects: Biochemistry, Genetics and Molecular Biology
Divisions: Faculty of Medicine > Basic Sciences > Applied Cell Sciences
Depositing User: ART . editor
Date Deposited: 10 Apr 2019 09:16
Last Modified: 10 Apr 2019 09:16
URI: http://eprints.kaums.ac.ir/id/eprint/4088

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