Association of Regulatory T Cells with Diabetes Type-1 and Its Renal and Vascular Complications Based on the Expression of Forkhead Box Protein P3 (FoxP3), Helios and Neurophilin-1

Khamechian, T. and Irandoust, B. and Mohammadi, H. and Nikoueinejad, H. and Akbari, H. (2018) Association of Regulatory T Cells with Diabetes Type-1 and Its Renal and Vascular Complications Based on the Expression of Forkhead Box Protein P3 (FoxP3), Helios and Neurophilin-1. Iranian Journal of Allergy, Asthma and Immunology, 17 (2). pp. 151-157.

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Abstract

In recent years, it has been recognized that regulatory T cells (Tregs) play a critical role in maintaining immune tolerance. Moreover, the expression of two markers named Helios and neurophilin-1 (NRP-1) has been highlighted in such cells. Helios, an intracellular transcription marker, largely differentiates twomost operative sub group of Tregs, namely naturally occurring (nTreg) and induced (iTreg) Tregs, and NRP-1 is reckoned as a membranous activity marker of Tregs. We aimed to count peripheral mononuclear cells expressing such markers in a group of type 1 diabetes patients to elucidate the possible role of Tregs in the pathogenesis of such disease and its complications. Blood samples from 61 adult patients with type 1 diabetes and 61 sex and age-matched healthy controls were tested to count two types of Tregs, namely naturally occurring and inducible types, according to the expression of cell surface markers of CD4/CD25/CD47�FITC/PE/APC and intracellular markers of FoxP3/Helios�PE-CY5/eFlour450 by flow cytometry, respectively.We also investigated the relation between expression of such markers with HbA1c, urine albumin/creatinine ratio (UACR), and common carotid intima thickness (CIMT). The circulatory frequency of both Helios+ and Helios- T-cells were significantly decreased in patients compared to those in healthy controls (p<0.001). There was also a significant decrease in circulatory frequency of Helios+ NRP-1+ and Helios- NRP-1+ cells in the patients compared to controls (p=0.029). According to expression of Helios and NRP-1 markers, the number and function of both Tregs were decreased in diabetic patients. Moreover, the neurophilin expression was inversely associated with complications of type 1 diabetes. Copyright© April 2018, Iran J Allergy Asthma Immunol. All rights reserved.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: CD4 antigen; CD47 antigen; helios; hemoglobin A1c; interleukin 2 receptor alpha; neuropilin 1; transcription factor; transcription factor FOXP3; unclassified drug, adult; arterial wall thickness; Article; controlled study; diabetic angiopathy; diabetic nephropathy; female; flow cytometry; human; human cell; insulin dependent diabetes mellitus; major clinical study; male; microalbuminuria; peripheral blood mononuclear cell; protein expression; regulatory T lymphocyte
Subjects: Pathology
Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Divisions: Faculty of Medicine > Basic Sciences > Department of Pathology& Histology
Depositing User: ART . editor
Date Deposited: 14 Apr 2019 09:29
Last Modified: 14 Apr 2019 09:29
URI: http://eprints.kaums.ac.ir/id/eprint/4045

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