Thermogel nanofiber induces human endometrial-derived stromal cells to neural differentiation: In vitro and in vivo studies in rat

Tavakol, S. and Aligholi, H. and Gorji, A. and Eshaghabadi, A. and Hoveizi, E. and Tavakol, B. and Rezayat, S.M. and Ai, J. (2014) Thermogel nanofiber induces human endometrial-derived stromal cells to neural differentiation: In vitro and in vivo studies in rat. Journal of Biomedical Materials Research - Part A, 102 (12). pp. 4590-4597.

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Abstract

Spinal cord injury (SCI) in humans remains a devastating and incurable disorder. The use of Matrigel, a hydrogelmimicking extracellular matrix, has been suggested as a scaffold for spinal cord regeneration. Human endometrial-derived stromal cells (hEnSCs) are abundant and available in adult stem cells with low immunological incompatibility, which could be considered for cell replacement therapy. The purpose of this study was to investigate the role ofMatrigel in neural differentiation of hEnSCs in vitro and assess the supportive effects of this hydrogel in an animal model of SCI. hEnSCs were isolated and encapsulated into nanofibrous thermogel and cell viability and cell membrane damage were assessed. Encapsulated hEnSCs into Matrigel were treated with neural differentiation medium for 21 days, and then neural genes and protein markers were analyzed using real time-PCR and immunocytochemistry. Matrigel was implanted into rats with SCI and followed for 42 days using a behavioral test. Our study revealed a higher cell viability and neural differentiation in the level of genes and proteins as well as lower cell membrane damage. Substantial recoveries of motor function were observed in animals receiving the Matrigel treatment. The treatment with Matrigel, nanofibrous scaffold, produced beneficial effects on functional recovery following SCI in rats, possibly via assimilation to cytoskeleton fiber, high surface/volume ratio, spatial interconnectivity and containing some adhesive molecules and growth factors, enhancement of antiinflammation, anti-astrogliosis, neuronal extension, and neuronal regeneration effects. © 2014 Wiley Periodicals, Inc.

Item Type: Article
Additional Information: cited By 11
Uncontrolled Keywords: Animals; Cell membranes; Cells; Cytology; Damage detection; Genes; Nanofibers; Neurons; Proteins; Rats; Recovery; Stem cells, Cell replacement therapy; Extracellular matrices; Matrigel; Motor neurons; Neural differentiations; Neuronal regenerations; Spinal cord injuries (SCI); Stromal cells, Molecular biology, brain derived neurotrophic factor; CD146 antigen; endoglin; epidermal growth factor; fibroblast growth factor 2; lactate dehydrogenase; matrigel; microtubule associated protein 2; nestin; oligodendrocyte transcription factor 2; Thy 1 antigen; nanofiber, animal experiment; animal model; animal tissue; Article; cell membrane; cell structure; cell viability; controlled study; cytoskeleton; endometrium cell; female; human; human cell; hydrogel; in vitro study; in vivo study; limb movement; locomotion; male; motor activity; motor performance; nerve cell differentiation; nerve regeneration; nonhuman; rat; real time polymerase chain reaction; spinal cord injury; stroma cell; tissue scaffold; adult; adult stem cell; animal; cell differentiation; chemistry; cytology; endometrium; metabolism; motoneuron; Spinal Cord Injuries; stem cell transplantation; stroma cell; transplantation; Wistar rat; xenograft, Adult; Adult Stem Cells; Animals; Cell Differentiation; Endometrium; Female; Heterografts; Humans; Male; Motor Neurons; Nanofibers; Rats; Rats, Wistar; Spinal Cord Injuries; Stem Cell Transplantation; Stromal Cells
Subjects: Biochemistry, Genetics and Molecular Biology
Divisions: Faculty of Medicine > Clinical Sciences > Department of Internal Medicine
Depositing User: editor . 2
Date Deposited: 07 Mar 2017 15:49
Last Modified: 07 Mar 2017 15:49
URI: http://eprints.kaums.ac.ir/id/eprint/403

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