A randomized double-blinded, placebo-controlled trial investigating the effect of fish oil supplementation on gene expression related to insulin action, blood lipids, and inflammation in gestational diabetes mellitus-fish oil supplementation and gestational diabetes

Jamilian, M. and Samimi, M. and Mirhosseini, N. and Ebrahimi, F.A. and Aghadavod, E. and Taghizadeh, M. and Asemi, Z. (2018) A randomized double-blinded, placebo-controlled trial investigating the effect of fish oil supplementation on gene expression related to insulin action, blood lipids, and inflammation in gestational diabetes mellitus-fish oil supplementation and gestational diabetes. Nutrients, 10 (2).

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Abstract

Gestational diabetes mellitus (GDM) is a common complication of pregnancy, and it is mostly associated with postpartum diabetes, insulin resistance, and dyslipidemia. Fish oil (omega-3) supplementation has been shown to reduce the risk of different chronic diseases such as cardiovascular disease, type 2 diabetes, and cancers, though the evidence of its impact on gestational diabetes is scarce. Our goal in this study was to determine the effect of fish oil administration on gene expression related to insulin action, blood lipids, and inflammation in women with GDM. Participants with GDM (n = 40), aged 18�40 years, were randomized to take either 1000 mg fish oil capsules, containing 180 mg eicosapentaenoic acid and 120 mg docosahexaenoic acid (n = 20), or placebo (n = 20) twice a day for 6 weeks. Gene expression related to insulin, lipids, and inflammation was quantified in peripheral blood mononuclear cells (PBMCs) of GDM women using Reverse Transcription Polymerase Chain Reaction (RT-PCR) method. Results of RT-PCR indicated that omega-3 supplementation upregulated gene expression of peroxisome proliferator-activated receptor gamma (PPAR-γ) (P = 0.04) in PBMCs of patients with GDM, compared with the placebo. In addition, gene expression of the low-density lipoprotein receptor (LDLR) (P < 0.001), interleukin-1 (IL-1) (P = 0.007), and tumor necrosis factor alpha (TNF-α) (P = 0.01) was downregulated in PBMCs of women with GDM, following omega-3 supplementation. No significant effect of omega-3 supplementation was indicated on gene expression of IL-8 in PBMCs of patients with GDM. Overall, fish oil supplementation for 6 weeks in women with GDM significantly improved gene expression of PPAR-γ, IL-1, and TNF-α, but not gene expression of IL-8. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.

Item Type: Article
Additional Information: cited By 3
Uncontrolled Keywords: docosahexaenoic acid; fish oil; icosapentaenoic acid; tumor necrosis factor; autacoid; biological marker; docosahexaenoic acid; icosapentaenoic acid; insulin; interleukin 1; LDLR protein, human; lipid; low density lipoprotein receptor; peroxisome proliferator activated receptor gamma; tumor necrosis factor, adult; Article; controlled study; double blind procedure; female; gene expression; human; inflammation; major clinical study; male; peripheral blood mononuclear cell; pregnancy diabetes mellitus; randomized controlled trial; real time polymerase chain reaction; reverse transcription polymerase chain reaction; blood; dietary supplement; gene expression regulation; genetics; Iran; metabolism; microcapsule; oral drug administration; pregnancy; pregnancy diabetes mellitus; time factor; treatment outcome, Administration, Oral; Adult; Biomarkers; Capsules; Diabetes, Gestational; Dietary Supplements; Docosahexaenoic Acids; Double-Blind Method; Eicosapentaenoic Acid; Female; Gene Expression Regulation; Humans; Inflammation Mediators; Insulin; Interleukin-1; Iran; Lipids; PPAR gamma; Pregnancy; Receptors, LDL; Time Factors; Treatment Outcome; Tumor Necrosis Factor-alpha
Subjects: Biochemistry, Genetics and Molecular Biology
Divisions: Faculty of Medicine > Basic Sciences > Department of Biochemistry
Depositing User: ART . editor
Date Deposited: 14 Apr 2019 10:58
Last Modified: 14 Apr 2019 10:58
URI: http://eprints.kaums.ac.ir/id/eprint/4010

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