The effects of probiotic supplementation on gene expression related to inflammation, insulin and lipid in patients with Parkinson�s disease: A randomized, double-blind, placebo-controlled trial

Borzabadi, S. and Oryan, S. and Eidi, A. and Aghadavod, E. and Kakhaki, R.D. and Tamtaji, O.R. and Taghizadeh, M. and Asemi, Z. (2018) The effects of probiotic supplementation on gene expression related to inflammation, insulin and lipid in patients with Parkinson�s disease: A randomized, double-blind, placebo-controlled trial. Archives of Iranian Medicine, 21 (7). pp. 289-295.

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Abstract

Background: This study was conducted to evaluate the effects of probiotic supplementation on gene expression related to inflammation, insulin and lipid in patients with Parkinson�s disease (PD).Methods: This randomized, double-blind, placebo-controlled clinical trial was conducted in 50 patients with PD as a pilot study. Participants were randomly allocated into two groups to take either 8�109 CFU/day probiotic supplements or placebo (n = 25 each group, one capsule daily) for 12 weeks. Gene expression related to inflammation, insulin, and lipid was quantified in peripheral blood mononuclear cells (PBMC) of PD patients, with RT-PCR method. Results: After the 12-week intervention, compared with the placebo, probiotic intake downregulated gene expression of interleukin-1 (IL-1) (P = 0.03), IL-8 (P < 0.001) and tumor necrosis factor alpha (TNF-α) (P=0.04) in PBMC of subjects with PD. In addition, probiotic supplementation upregulated transforming growth factor beta (TGF-β) (P = 0.02) and peroxisome proliferator-activated receptor gamma (PPAR-γ) (P = 0.03) in PBMC of subjects with PD compared with the placebo. We did not observe any significant effect of probiotic intake on gene expression of low-density lipoprotein receptor (LDLR) and vascular endothelial growth factor (VEGF) in PBMC of patients with PD. Conclusion: Overall, probiotics supplementation for 12 weeks in PD patients significantly improved gene expression of IL-1, IL-8, TNF-α, TGF-β and PPAR-γ, but did not affect gene expression of VEGF and LDLR, and biomarkers of inflammation and oxidative stress. © 2018 The Author(s).

Item Type: Article
Additional Information: cited By 3
Uncontrolled Keywords: biological marker; complementary DNA; glutathione; glutathione peroxidase 3; insulin; interleukin 1; interleukin 8; lactocare; lipid; low density lipoprotein receptor; peroxisome proliferator activated receptor gamma; placebo; probiotic agent; trace element; transforming growth factor beta; tumor necrosis factor; vasculotropin, adult; aged; Article; Bifidobacterium bifidum; blood biochemistry; body mass; clinical article; clinical trial; controlled clinical trial; controlled study; diet supplementation; double blind procedure; down regulation; drug effect; female; gene expression; human; inflammation; Lactobacillus acidophilus; Lactobacillus fermentum; Lactobacillus reuteri; lymphocyte; male; medication compliance; oxidative stress; Parkinson disease; patient compliance; peripheral blood mononuclear cell; physical activity; pilot study; randomized controlled trial; real time polymerase chain reaction; upregulation
Subjects: Biochemistry, Genetics and Molecular Biology
Divisions: Faculty of Medicine > Basic Sciences > Department of Biochemistry
Depositing User: ART . editor
Date Deposited: 17 Apr 2019 07:53
Last Modified: 17 Apr 2019 07:53
URI: http://eprints.kaums.ac.ir/id/eprint/3890

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