IL-1RA VNTR and IL-1α 4845G>T polymorphisms and risk of idiopathic male infertility in Iranian men: A case�control study and an in silico analysis

Zamani-Badi, T. and Nikzad, H. and Karimian, M. (2018) IL-1RA VNTR and IL-1α 4845G>T polymorphisms and risk of idiopathic male infertility in Iranian men: A case�control study and an in silico analysis. Andrologia, 50 (9).

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Abstract

This study aimed to investigate the association of IL-1RA VNTR and IL-1α 4845G>T polymorphisms with idiopathic male infertility followed by an in silico analysis. In a case�control study, we collected blood samples from 230 infertile and 230 healthy men. Genotyping of IL-1RA VNTR was performed by PCR whereas IL-1α 4845G>T was genotyped by polymerase chain reaction-restriction fragment length polymorphism. An in silico approach was employed for the detection of IL-1RA VNTR and IL-1α 4845G>T effects on some molecular aspects of IL-1RA and IL-1α respectively. The result of our genetic association study for IL-1α 4845G>T revealed that there was a significant association between GT genotype, TT genotype, T allele and idiopathic male infertility. Although there was no significant association between IL-1RA VNTR and male infertility in the overall analysis. However, subgroup analysis revealed that the subjects with VNTR 4R/5R genotype were at a higher risk of oligozoospermia. Furthermore, 4845TT genotype, and 4845T allele were associated with oligozoospermia, asthenozoospermia and nonobstructive azoospermia. Bioinformatics analysis showed that IL-1RA VNTR may affect the splicing pattern of IL-1RA. Moreover, IL-1α 4845G>T has a significant effect on RNA structure and protein function. Based on our findings, both IL-1RA VNTR and IL-1α 4845G>T polymorphisms could be considered as potential biomarkers for screening of susceptible individuals. © 2018 Blackwell Verlag GmbH

Item Type: Article
Additional Information: cited By 3
Uncontrolled Keywords: biological marker; genomic DNA; interleukin 1; interleukin 1 receptor blocking agent; IL1A protein, human; IL1RN protein, human; interleukin 1 receptor blocking agent; interleukin 1alpha, adult; Article; asthenospermia; azoospermia; case control study; computer model; controlled study; DNA sequence; genotype; human; idiopathic disease; Iranian (citizen); major clinical study; male; male infertility; oligospermia; polymerase chain reaction; protein secondary structure; restriction fragment length polymorphism; screening; single nucleotide polymorphism; variable number of tandem repeat; amino acid sequence; chemistry; computer simulation; genetics; Iran; male infertility, Adult; Amino Acid Sequence; Case-Control Studies; Computer Simulation; Humans; Infertility, Male; Interleukin 1 Receptor Antagonist Protein; Interleukin-1alpha; Iran; Male; Minisatellite Repeats; Polymorphism, Single Nucleotide
Subjects: Anatomy Morphology
Biochemistry, Genetics and Molecular Biology
Divisions: Faculty of Medicine > Basic Sciences > Applied Cell Sciences
Depositing User: ART . editor
Date Deposited: 23 Apr 2019 09:28
Last Modified: 23 Apr 2019 09:28
URI: http://eprints.kaums.ac.ir/id/eprint/3809

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