Lethal effects of gold nanoparticles on protoscolices of hydatid cyst: in vitro study

Napooni, S. and Arbabi, M. and Delavari, M. and Hooshyar, H. and Rasti, S. (2019) Lethal effects of gold nanoparticles on protoscolices of hydatid cyst: in vitro study. Comparative Clinical Pathology, 28 (1). pp. 143-150.

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Abstract

Recurrence of hydatidosis may results from cyst fluid spillage during surgery. To inactivate the cyst content, different scolicidal agents have been introduced. Nevertheless, novel and more effective treatments are needed due to the associated complications. In the current study, we examined the scolicidal effects of gold nanoparticles on Echinococcus granulosus protoscoleces. Hydatid cysts of sheep liver were collected in this study. The cyst fluid containing protoscoleces was aspirated aseptically. The protoscoleces were exposed to gold nanoparticles at different concentrations (250, 500, 1000, 2000, and 4000 μg/mL). Eosin staining method was applied to determine the viability of protoscoleces at 5, 10, 20, 30, and 60 min. Moreover, cytotoxicity analysis, scanning electron microscopy (SEM) of ultrastructural changes, DNA fragmentation, were performed, and protoscoleces were studied following treatment. The significant scolicidal effects of gold nanoparticles were observed at all concentrations compared to the control group. A total of 4000 μg/mL of gold nanoparticles could destroy 76 of protoscoleces in 60 min. Cytotoxic effects of gold nanoparticle on J774 macrophage cell line, in minimum and maximum concentration (500 and 4000 μg/mL), were 1 and 11, respectively. After treatment of protoscoleces with gold nanoparticles in different times and concentrations, considerable alteration in size, ultrastructure changes in tegument and shape of sucker, as well as DNA fragmentation of parasite were seen. Based on the results, gold nanoparticles could exert scolicidal effects on hydatid cyst protoscoleces; therefore, they can be applied in hydatid cyst treatment. © 2018, Springer-Verlag London Ltd., part of Springer Nature.

Item Type: Article
Additional Information: cited By 0
Subjects: Parasitology
Divisions: Faculty of Medicine > Basic Sciences > Department of Parasitological & Mycology
Depositing User: ART . editor
Date Deposited: 11 May 2019 10:59
Last Modified: 11 May 2019 10:59
URI: http://eprints.kaums.ac.ir/id/eprint/3713

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