Berberine ameliorates experimental varicocele-induced damages at testis and sperm levels; evidences for oxidative stress and inflammation

Hassani-Bafrani, H. and Najaran, H. and Razi, M. and Rashtbari, H. (2019) Berberine ameliorates experimental varicocele-induced damages at testis and sperm levels; evidences for oxidative stress and inflammation. Andrologia, 51 (2).

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DOI: UNSPECIFIED

Abstract

The present study was performed to show the ameliorative effect of berberine (BBR), as an antioxidant and anti-inflammatory agent, against experimental varicocele (VCL)-induced molecular and histological damages. For this purpose, 50 mature Wistar rats were divided into control, control-sham, VCL-sole, 50 mg/kg and 100 mg/kg BBR-treated VCL-induced groups. The tissue levels of interleukin-6 (IL-6), tumour necrosis factor-α (TNF-α), nitric oxide (NO), total antioxidant capacity (TAC), malondialdehyde (MDA), superoxide dismutase (SOD) and gluthatione peroxidase (GSH-px) as well as the mRNA levels of testicular CuZn SOD, MnSOD, EC-SOD and GSH-px were evaluated. The serum concentration of testosterone and germ cells mRNA damage were analysed. Finally, the sperm viability, motility, DNA integrity and chromatin condensation were analysed. Observations revealed that, the BBR significantly downregulated VCL-increased IL-6, TNF-α and NO levels, upregulated the CuZn SOD, MnSOD, EC-SOD and GSH-px mRNA level, decreased testicular MDA content, enhanced serum testosterone level and ameliorated testicular TAC, SOD and GSH-px levels. The animals in BBR-treated groups exhibited diminished mRNA damage versus non-treated VCL-induced group. The BBR has significantly (p < 0.05) improved sperm parameters. In conclusion, the BBR by promoting testicular antioxidant potential and by downregulating inflammatory reactions fairly promotes spermatogenesis and upregulates the sperm quality. © 2018 Blackwell Verlag GmbH

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: berberine; copper zinc superoxide dismutase; extracellular superoxide dismutase; glutathione peroxidase; interleukin 6; malonaldehyde; manganese superoxide dismutase; messenger RNA; nitric oxide; testosterone; tumor necrosis factor, animal experiment; animal model; antioxidant activity; Article; chromatin condensation; controlled study; DNA fragmentation; down regulation; enzyme linked immunosorbent assay; germ cell; immunohistochemistry; inflammation; male; nonhuman; oxidative stress; rat; seminiferous tubule; sperm; sperm quality; spermatogenesis; spermatozoon count; spermatozoon motility; testis; testosterone blood level; treatment duration; varicocele
Subjects: Medicine
Agricultural and Biological Sciences
Divisions: Faculty of Medicine > Basic Sciences > Department of Anatomy
Depositing User: ART . editor
Date Deposited: 22 May 2019 14:43
Last Modified: 22 May 2019 14:43
URI: http://eprints.kaums.ac.ir/id/eprint/3694

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