Specific fluorometric assay for direct determination of amikacin by molecularly imprinting polymer on high fluorescent g-C 3 N 4 quantum dots

Hassanzadeh, J. and Moghadam, B.R. and Sobhani-Nasab, A. and Ahmadi, F. and Rahimi-Nasrabadi, M. (2019) Specific fluorometric assay for direct determination of amikacin by molecularly imprinting polymer on high fluorescent g-C 3 N 4 quantum dots. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 214. pp. 451-458.


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Here, a specific and reliable fluorometric method for the rapid determination of amikacin was developed based on the molecularly imprinting polymer (MIP) capped g-C 3 N 4 quantum dots (QDs). g-C 3 N 4 QDs were obtained by facile and one-spot ethanol-thermal treatment of bulk g-C 3 N 4 powder and showed a high yield fluorescence emission under UV irradiation. The MIP layer was also created on the surface on QDs, via usual self-assembly process of 3-aminopropyl triethoxysilane (APTES) functional monomers and tetraethyl ortho-silicate (TEOS) cross linker in the presence of amikacin as template molecules. The synthesized MIP-QDs composite showed an improved tendency toward the amikacin molecules. In this state, amikacin molecules located adjacent to the g-C 3 N 4 QDs caused a remarkable quenching effect on the fluorescence emission intensity of QDs. This effect has a linear relationship with amikacin concentration and so, formed the basis of a selective assay to recognize amikacin. Under optimized experimental conditions, a linear calibration graph was obtained as the quenched emission and amikacin concentration, in the range of 3�400 ng mL �1 (4.4�585.1 nM) with a detection limit of 1.2 ng mL �1 (1.8 nM). The high selectivity of MIP sites as well as individual fluorescence properties of g-C 3 N 4 QDs offers a high specific and sensitive monitoring method for drug detection. The method was acceptably applied for the measurement of amikacin in biological samples. © 2019 Elsevier B.V.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: Fluorescence; Irradiation; Molecules; Nanocrystals; Polymers; Self assembly; Silicates; Synthesis (chemical), Amikacin; Experimental conditions; Fluorescence emission; Fluorescence emission intensity; Fluorescence properties; Molecularly imprinting polymers; Self assembly process; Tetra-ethyl-ortho-silicate, Semiconductor quantum dots
Subjects: Chemistry
Pharmacology, Toxicology and Pharmaceutics
Divisions: Faculty of Medicine > Basic Sciences > Department of Pharmacology
Depositing User: ART . editor
Date Deposited: 01 May 2019 14:33
Last Modified: 01 May 2019 14:33
URI: http://eprints.kaums.ac.ir/id/eprint/3660

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