The LCN2-engineered HEK-293 cells show senescence under stressful condition

Bahmani, B. and Amiri, F. and Mohammadi-Roushandeh, A. and Bahadori, M. and Dehghan-Harati, M. and Habibi-Roudkenar, M. (2015) The LCN2-engineered HEK-293 cells show senescence under stressful condition. Iranian Journal of Basic Medical Sciences, 18 (5). pp. 459-464.

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Abstract

Objective(s): Lipocalin2 (Lcn2) gene is highly expressed in response to various types of cellular stresses. The precise role of Lcn2 has not been fully understood yet. However, it plays a key role in controlling vital cellular processes such as proliferation, apoptosis and metabolism. Recently it was shown that Lcn2 decreases senescence and increases proliferation of mesenchymal stem cells (MSC) with finite life span under either normal or oxidative stress conditions. However, Lcn2 effects on immortal cell line with infinite proliferation are not defined completely. Materials and Material and Methods: HEK-293 cells were transfected with recombinant pcDNA3.1 containing Lcn2 fragment (pcDNA3.1-Lcn2). Expression of lipocalin2 in transfected cells was evaluated by RT-PCR, real time RT-PCR, and ELISA. Different cell groups were treated with H2O2 and WST-1 assay was performed to determine their proliferation rate. Senescence was studied by β-galactosidase and gimsa staining methods as well as evaluation of the expression of senescence-related genes by real time RT-PCR. Results: Lcn2 increased cell proliferation under normal culture condition, while the proliferation slightly decreased under oxidative stress. This decrease was further found to be attributed to senescence. Conclusion: Our findings indicated that under harmful conditions, Lcn2 gene is responsible for the regulation of cell survival through senescence. © 2015, Iranian Journal of Basic Medical Sciences. All rights reserved.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: beta galactosidase; hydrogen peroxide; neutrophil gelatinase associated lipocalin; Taq polymerase, Article; cell proliferation; cell proliferation assay; enzyme linked immunosorbent assay; gene; gene expression; genetic transfection; human; human cell; Lcn2 gene; mesenchymal stem cell; molecular cloning; oxidative stress; real time polymerase chain reaction; reverse transcription polymerase chain reaction; senescence; stress; upregulation
Subjects: Biochemistry, Genetics and Molecular Biology
Divisions: Faculty of Medicine > Basic Sciences > Department of Anatomy
Depositing User: editor . 2
Date Deposited: 07 Mar 2017 19:01
Last Modified: 07 Mar 2017 19:01
URI: http://eprints.kaums.ac.ir/id/eprint/364

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