Molecular assessment of Neospora caninum and Toxoplasma gondii in hooded crows (Corvus cornix) in Tehran, Iran

Abdoli, A. and Arbabi, M. and Pirestani, M. and Mirzaghavami, M. and Ghaffarifar, F. and Dalimi, A. and Sadraei, J. (2018) Molecular assessment of Neospora caninum and Toxoplasma gondii in hooded crows (Corvus cornix) in Tehran, Iran. Comparative Immunology, Microbiology and Infectious Diseases, 57. pp. 69-73.

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Abstract

Abstract Neospora caninum and Toxoplasma gondii are two closely related protozoan parasites that have been detected from various species of bird hosts. However, little is known about the prevalence of N. caninum and T. gondii in crows. Hence, we examined the molecular frequency of N. caninum and T. gondii in the brain samples of hooded crows (Corvus cornix) that collected from different public parks of Tehran, Iran by nested-PCR method. We used the primers targeting the Nc5 and GRA6 genes for detection of N. caninum and T. gondii, respectively. From a total of 55 brain samples, 5 (9.9%) and 9 (16.36%) samples were positive for N. caninum and T. gondii, respectively. Sequencing of a N. caninum isolate revealed 95%–100% identity with the deposited N. caninum in GenBank. Genotyping of T. gondii isolates by PCR-RFLP analysis of the GRA6 gene revealed type III genotype in 8 isolates. The results of this study indicate that hooded crows may have a putative role in transmission of N. caninum and T. gondii to canines and felines definitive hosts, respectively.

Item Type: Article
Additional Information: cited By 0
Subjects: Parasitology
Divisions: Faculty of Medicine > Basic Sciences > Department of Parasitological & Mycology
Depositing User: ART . editor
Date Deposited: 13 Feb 2019 13:31
Last Modified: 13 Feb 2019 13:31
URI: http://eprints.kaums.ac.ir/id/eprint/3407

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