Speciation of As()/As(V) and Total Inorganic Arsenic in Biological Fluids Using New Mode of Liquid-Phase Microextraction and Electrothermal Atomic Absorption Spectrometry

Haghnazari, L. and Mirzaei, N. and Arfaeinia, H. and Karimyan, K. and Sharafi, H. and Fattahi, N. (2018) Speciation of As()/As(V) and Total Inorganic Arsenic in Biological Fluids Using New Mode of Liquid-Phase Microextraction and Electrothermal Atomic Absorption Spectrometry. Biological Trace Element Research, 183 (1). pp. 173-181.

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Abstract

Abstract In this paper, a new extraction method based on countercurrent liquid–liquid microextraction (CLLME) has been developed for the extraction and preconcentration of inorganic arsenic (iAs) in plasma and urine samples prior to their analysis by electrothermal atomic absorption spectrometry (ETAAS). In this method, firstly, 5 ml of water is added to the extraction vessel. Then 30.0 μl of the extracting solvent is added to it in order for the extracting solvent to be placed in the narrow-necked vessel. In total, 10 ml of a standard solution or a pretreated real sample is added to the sample container and it is connected to the extraction vessel via a connector. While opening the embedded valve at the bottom of the sample container and the one in the extraction vessel, the sample solution flows into the extracting solvent with the same flow rate, leading to the successful extraction of metal ligand into the extracting organic solvent. Under the optimum conditions, calibration curves are linear in the range of 0.1–50 μg l−1, and limit of detections (LODs) are in the range of 0.03–0.05 μg l−1. The enhancement factor and enrichment factor were in the range of 220–240 and 198–212, respectively. Repeatability (intra-day) and reproducibility (inter-day) of method based on seven replicate measurements of 5.0 μg l−1 of arsenic were in the range of 2.3–3.5% and 4.0–5.7%, respectively. The applicability of the proposed CLLME and ETAAS methods was demonstrated by analyzing the iAs in spiked urine and plasma samples. The obtained recoveries of the arsenic in the range of 92–107% indicated the excellent capability of the developed method for speciation of arsenic from plasma and urine samples. Keywords: Arsenic speciation Countercurrent liquid–liquid microextraction Extraction solvent lighter than water Biological analysis

Item Type: Article
Additional Information: cited By 2
Subjects: Health Professions
Biochemistry, Genetics and Molecular Biology
Divisions: Faculty of Health > Department of Environmental health
Depositing User: ART . editor
Date Deposited: 27 Feb 2019 12:16
Last Modified: 27 Feb 2019 12:16
URI: http://eprints.kaums.ac.ir/id/eprint/3396

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