Neuroprotective Effects of Oxytocin Hormone after an Experimental Stroke Model and the Possible Role of Calpain-1

Etehadi Moghadam, S. and Azami Tameh, A. and Vahidinia, Z. and Atlasi, M.A. and Hassani Bafrani, H. and Naderian, H. (2018) Neuroprotective Effects of Oxytocin Hormone after an Experimental Stroke Model and the Possible Role of Calpain-1. Journal of Stroke and Cerebrovascular Diseases, 27 (3). pp. 724-732.

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Abstract

Background Different mechanisms will be activated during ischemic stroke. Calpain proteases play a pivotal role in neuronal death after ischemia damage through apoptosis. Anti-apoptotic activities of the oxytocin (OT) in different ischemic tissues were reported in previous studies. Recently, a limited number of studies have noted the protective effects of OT in the brain. In the present study, the neuroprotective potential of OT in an animal model of transient middle cerebral artery occlusion (tMCAO) and the possible role of calpain-1 in the penumbra region were assessed. Methods Adult male Wistar rats underwent 1 hour of tMCAO and were treated with nasal administration of OT. After 24 hours of reperfusion, infarct size was evaluated by triphenyltetrazolium chloride. Immunohistochemical staining and Western blotting were used to examine the expression of calpain-1. Nissl staining was performed for brain tissue morphology evaluation. Results OT reduced the infarct volume of the cerebral cortex and striatum compared with the ischemia control group significantly (P < .05). Calpain-1 overexpression, which was caused by ischemia, decreased after OT administration (P < .05). The number of pyknotic nuclei in neurons increased dramatically in the ischemic area and OT attenuated the apoptosis of neurons in the penumbra region (P < .01). Conclusion We provided evidence for the neuroprotective role of OT after tMCAO through calpain-1 attenuation. Key Words Stroke tMCAO calpain-1 oxytocin

Item Type: Article
Additional Information: cited By 1
Subjects: Anatomy Morphology
Endocrinology Metabolism
Divisions: Faculty of Medicine > Basic Sciences > Department of Anatomy
Depositing User: ART . editor
Date Deposited: 05 Mar 2019 12:53
Last Modified: 05 Mar 2019 12:53
URI: http://eprints.kaums.ac.ir/id/eprint/3294

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