Study of antibiotic resistance pattern and incidence of pathogenic genes of mgtC, spi4R, agfA, invE/A and ttrC in Salmonella infantis isolated from clinical specimens

Aghdasi-Araghinezhad, R. and Amini, K. (2017) Study of antibiotic resistance pattern and incidence of pathogenic genes of mgtC, spi4R, agfA, invE/A and ttrC in Salmonella infantis isolated from clinical specimens. Feyz Journal of Kashan University of Medical Sciences, 21. pp. 442-449.

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Official URL: http://feyz.kaums.ac.ir/article-1-3178-en.html
DOI: UNSPECIFIED

Abstract

Background: The importance of the health of red meat, poultry and eggs in human nutrition is very high. One of the factors that jeopardize the health of poultry food products is the bacterial family of Enterobacteriaceae, especially Salmonella. The aim of this study was to detect pathogenic genes in Salmonella infectious bacteria isolated from stool specimens using the multiple PCR assay. Materials and Methods: Selective and specific media for isolation of Salmonella were used. Primary isolation was carried out using Peptone water, Rapaport, selenite cysteine, MacConky agar and xylose-lysine deoxycholate agar. To confirm the diagnosis, biochemical tests including TSI, urea, endodontic, and citrate were used. The Salmonella Polyvalent Kit was used to determine Salmonella groups and mgtC, spi4R, agfA, invE/A and ttrC genes were studied in 60 samples by the multiple PCR method. Results: The results showed that all samples had 2 genes mgtC and ttrC, and none of the samples showed resistance to cefepime. Of the 60 samples of Salmonella, none were resistant to cefepime and ceftriaxone; 38.8 of the samples were resistant to amoxicillin, 53 to erythromycin and 38.3 to sulfamethoxazole. Conclusion: It can be concluded that cefepime is the best selective drug for the treatment of Salmonella infections. Identification and validation of genes in the region's bacteria can play a role in the broad epidemiological examination, antibiotic resistance, vaccine production, level of virulence, prevention and treatment. Also, evaluation of these genes in the samples for their virulence index is very important.

Item Type: Article
Subjects: Biochemistry, Genetics and Molecular Biology
Divisions: Feyz journal
Depositing User: editor . feyz
Date Deposited: 25 Nov 2017 17:41
Last Modified: 25 Nov 2017 17:41
URI: http://eprints.kaums.ac.ir/id/eprint/3084

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