Characterization of CTX-M-type extend-spectrum lactamase producing Klebsiella spp. in Kashan, Iran

Afzali, H. and Firoozeh, F. and Amiri, A. and Moniri, R. and Zibaei, M. (2015) Characterization of CTX-M-type extend-spectrum lactamase producing Klebsiella spp. in Kashan, Iran. Jundishapur Journal of Microbiology, 8 (10). pp. 1-5.

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Context: The CTX-M family consists of more than 50 β-lactamases, which are grouped on the basis of sequences into five subtypes including CTX-M-1, CTX-M-2, CTX-M-8, CTX-M-9 and CTX-M-25. Objectives: The current study aimed to detect subtypes of CTX-M extended-spectrum β-lactamases (ESBLs) among ESBL positive Klebsiella isolates from patients in Kashan, Iran. Materials and Methods: A total of 100 clinical isolates of Klebsiella were collected and the isolates, which showed resistance or reduced susceptibility to cefotaxime, ceftazidime and/or aztreonam by the disk diffusion method were selected. These isolates were identified as ESBL-producing isolates by double disk synergy tests using clavulanic acid, cefotaxime, ceftazidime and aztreonam. The blaCTX-M type determinants were identified by the Polymerase Chain Reaction (PCR) method followed by DNA sequencing. Results: Of the 100 Klebsiella isolates, 41 (41) demonstrated resistance or reduced susceptibility to ceftazidime and/or aztreonam and 35 (n = 35) were ESBL-producers. Twenty-eight (8o) of the ESBL-producing isolates carried the blaCTX-M type genes. Based on PCR assays and sequencing of blaCTX-M genes, CTX-M-1, CTX-M-2 and CTX-M-9 were identified in 21 (60), 15 (42) and nine (34) of these isolates, respectively (GenBank accession numbers KJ803828-KJ803829). Conclusions: Our study showed that the frequency of blaCTX-M genes among Klebsiella isolates in our region is at an alarming rate. Also, we found a high prevalence of blaCTX-M-1 β-lactamase in Klebsiella isolates in Kashan. © 2015, Ahvaz Jundishapur University of Medical Sciences.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: amoxicillin plus clavulanic acid; ampicillin; aztreonam; beta lactamase CTX M; beta lactamase CTX M 1; beta lactamase CTX M 2; beta lactamase CTX M 9; cefalotin; cefixime; cefotaxime; cefoxitin; ceftazidime; ciprofloxacin; cotrimoxazole; extended spectrum beta lactamase; gentamicin; imipenem; nalidixic acid; nitrofurantoin; unclassified drug, antibiotic resistance; Article; bacterial gene; bacterium identification; bacterium isolate; blaCTX M gene; controlled study; disk diffusion; enzyme synthesis; female; gene sequence; human; Iran; Klebsiella; mouse; nonhuman
Subjects: Immunology and Microbiology
Divisions: Faculty of Medicine > Basic Sciences > Department of Microbiology & Immunology
Depositing User: editor . 2
Date Deposited: 08 Mar 2017 17:05
Last Modified: 08 Mar 2017 17:05

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