Comparison of in vitro culture of preantral follicle isolated from vitrified-warmed mouse ovaries with fresh follicles in culture media

Mazoochi, T. and Salehnia, M. and Rezazadeh Valojerdi, M. and Mowla, S.J. and Hajizadeh, E. (2007) Comparison of in vitro culture of preantral follicle isolated from vitrified-warmed mouse ovaries with fresh follicles in culture media. Feyz Journal of Kashan University of Medical Sciences, 11.

Full text not available from this repository.
Official URL: http://feyz.kaums.ac.ir/article-1-56-en.html
DOI: UNSPECIFIED

Abstract

Background: Vitrification is a simple and ultra rapid technique for the conservation of fertility. In this study, we compare the in vitro development of preantral follicles obtained from fresh specimens with vitrified- warmed mouse ovaries.Materials and Methods: This experimental study was carried out on 20, 14-day-old female mice (NMRI). Ovaries were vitrified with a solution containing ethylene glycol, ficoll 70 and sucrose in PB1 (EGFS40) for 5 min, and transferred directly into liquid nitrogen and stored for one week. Fast warming was done by descending sucrose at room temperature. Preantral follicles with 100-130 µm in diameter were mechanically isolated from fresh and vitrified-warmed ovaries and cultured in α-minimum essential medium (α-MEM) supplemented with 5 fetal bovine serum (FBS), 100 mIU/ml rFSH, 1 ITS, and 20ng/ml mrEGF in vitro for 10 days. Diameter of follicle, and, survival rate and number of antral follicles in both groups were compared using t-test and chi-square test, respectively.Results: Isolated follicles from the vitrified and nonvitrified groups survived and grew in vitro culture. On the day 6 survival rates in the vitrified and fresh control groups were 72.1 and 78.6, and, on the day 10, they were 66.9 and 72.6, respectively. Follicle antrum formation was 37.5 in the vitrified group while it was 43.5 in the fresh group in the 10th day. On the day 2, the mean diameters of fresh and vitrified follicles were 158.11 ± 11.23 and 155.48 ± 8.35 and on the day 4, they were 201.56 ± 9.87 and 193.42 ± 8.46, respectively. There was no significant difference between the control and vitrified groups in these variables (p>0.05).Conclusion: Taken together, cryopreservation of the ovary by vitrification seems to be a promising method to preserve ovarian follicles.

Item Type: Article
Subjects: Medicine
Divisions: Feyz journal
Depositing User: ART . editor
Date Deposited: 11 May 2017 15:23
Last Modified: 03 Jun 2017 11:55
URI: http://eprints.kaums.ac.ir/id/eprint/2136

Actions (login required)

View Item View Item