Effect of vitrification with single agent in stepwise manner using different concentrations of ethylene gycol on the morphology of mouse ovarian tissue

Mirrasekhian, E. and Salehnia, M. (2007) Effect of vitrification with single agent in stepwise manner using different concentrations of ethylene gycol on the morphology of mouse ovarian tissue. Feyz Journal of Kashan University of Medical Sciences, 11.

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Official URL: http://feyz.kaums.ac.ir/article-1-32-en.html
DOI: UNSPECIFIED

Abstract

Background: Considering the importance of cryoprotection and dehydration manner in the vitrification procedure, the aim of this study was to evaluate the effect of this technique using different concentrations of ethylene glycol (EG) as a single agent in stepwise manner and different dehydration time on the mouse ovarian tissue. Materials and Methods: Thirty five NMRI adult mice (6-10 weeks) were sacrificed and their ovarian tissues were divided into control, toxicity test and vitrified groups. After dehydration of samples in increasing concentration of EG (2 and 4 M) for 1 min in every group the vitrification procedure was done using final concentration of 6 or 8 M of EG in different times (4,6, 8 min). The ovarian tissue in each vitrified group was stored in liquid nitrogen for one week. After warming, the samples were washed in 1 M sucrose solution. The samples of fresh, vitrified and toxicity test groups were fixed in 10 formaldehyde solution and embedded in paraffin. Then serial sections were prepared, stained with hematoxylen and eosin and studied morphologically. Results: The morphology of ovarian tissue in all groups with different concentrations of EG and different dehydration times was almost the same. The preantral and antral follicles were well preserved especially on the cortical part of ovary they had a normal morphology. Conclusion: The results showed that high concentration of EG (6 or 8 M) was not toxic and dehydration time from 4 to 8 minutes had no harmful effect on the structure of ovarian tissue during vitrification and warming. However, more additional investigations are recommended.

Item Type: Article
Subjects: Medicine
Divisions: Feyz journal
Depositing User: ART . editor
Date Deposited: 11 May 2017 15:01
Last Modified: 03 Jun 2017 12:50
URI: http://eprints.kaums.ac.ir/id/eprint/2124

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