Haghdoost-Yazdi, H. and Esmaeili, M.R. and Sophiabadi, M.R. and Azhdari-Zarmehri, H. and Janahmadi, M. (2010) The role of potassium channels in the regulation of calcium spike configuration in the cerebellar Purkinje neurons. Feyz Journal of Kashan University of Medical Sciences, 14.
Full text not available from this repository.Abstract
Background: Calcium spikes play important roles in the control of neuronal spontaneous activity. Cerebellar Purkinje neurons fire spontaneous calcium spikes. In this study, the role of potassium channels in the regulation of these spikes was studied. Materials & Methods: Brain slices from the cerebellum of young rats were prepared and the Purkinje cells were visualized using an upright microscope. Using borosilicate micropipettes and Axoclamp 2B amplifier, intracellular recordings were taken from the cells. The role of different K+ channels in the regulation of calcium spikes configuration was determined using different K+ channels blockers and precise analysis of the recorded calcium spikes in the presence of blockers. Results: Application of wide-range 4-aminophyridine and tetraethylammonium blockers increased duration and amplitude of afterhyperpolarization (AHP) of the calcium spikes and converted them from one-peak spike to two- or multiple-peaks spikes. Blockade of small conductance calcium dependent potassium channels increased duration of the spikes but had no effect on the AHP amplitude Blockade of large conductance calcium dependent potassium channels increased duration of the calcium spikes and decreased the AHP amplitude. Conclusion: Our results showed that properties of the calcium spikes in the cerebellar Purkinje neurons were largely controlled by different potassium channels, including calcium dependent types.
| Item Type: | Article |
|---|---|
| Subjects: | Neuroscience |
| Divisions: | Feyz journal |
| Depositing User: | ART . editor |
| Date Deposited: | 10 May 2017 13:10 |
| Last Modified: | 24 May 2017 19:00 |
| URI: | http://eprints.kaums.ac.ir/id/eprint/1969 |
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