The interaction between harmane and nicotinic receptors of dorsal hippocampus in a hole-board test of anxiety in mice

Piri, M. and Nasehi, M. and Shahin, M.S. and Zarrindast, M.R. (2011) The interaction between harmane and nicotinic receptors of dorsal hippocampus in a hole-board test of anxiety in mice. Feyz Journal of Kashan University of Medical Sciences, 14.

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Official URL: http://feyz.kaums.ac.ir/article-1-1044-en.html
DOI: UNSPECIFIED

Abstract

Background: β-carboline alkaloids, such as harmane, are found in common plant-derived foodstuffs and plant-derived inhalation components of tobacco. In the present study, the involvement of dorsal hippocampus nicotinic receptor in the harmane effects on anxiety behavior has been evaluated. Materials and Methods: Mice were anesthetized with an intra-peritoneal injection of ketamine hydrochloride plus xylazine and then placed in a stereotaxic apparatus. Cannual were bilaterally implanted in the CA1 region of hippocampus. All animals were allowed to recover for 1 week before the beginning of the behavioral testing. The hole-board test was used to evaluate the anxiety-like behaviors. One-way analys was of variance so that Dunnett’s test was used to analyse data. All experiments were performed in accordance with institutional guidelines for animal care and use. Results: Intraperitoneal injection of harmane decreased the number of head dip and locomotion (P<0.001). While bilateral intra-dorsal hippocampal injections of nicotine decreased the number of head dip (P<0.01), it had no effect on locomotor activity. Furthermore, intra-dorsal hippocampal injection of mecamylamine (nicotinic receptor antagonist) in the presence and absence of harmane had no effect on anxiety behavior and locomotion (P>0.05). Conclusion: harmane and nicotine not only display anxiogenic effects but also demonstrate a complex interaction. The findings also indicated that harmane induces anxiety via non-nicotinic receptors.

Item Type: Article
Subjects: Neuroscience
Divisions: Feyz journal
Depositing User: ART . editor
Date Deposited: 10 May 2017 13:00
Last Modified: 31 May 2017 12:22
URI: http://eprints.kaums.ac.ir/id/eprint/1959

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