Evaluation of the effect of adipose tissue-derived stem cells on the quality of bone healing around implants

Golab, K.G. and Ragerdi-Kashani, I. and Azami-Tameh, A. and Zaminy, A. and Namjoy-Nik, I. and Namjoy-Nik, S. (2016) Evaluation of the effect of adipose tissue-derived stem cells on the quality of bone healing around implants. Connective Tissue Research, 57 (1). pp. 10-19.

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Abstract

Purpose/Aim: This study evaluates the efficacy of grafted adipose-derived stem cells (ADSCs) on blade-type implants in improving osseointegration in rat femurs using a low-densitybone model. Materials and Methods: After isolating and expanding ADSCs, twice-passaged cells were seeded on blade-type implants on culture plates. Osteogenic induction of grafted cells began after attaching cells to the prepared titanium surfaces and it continued for 4 days. The scaffolds were then implanted in the femurs of Wistar rats. Osteogenic differentiation of these cells was confirmed using polymerase chain reaction (PCR) and alizarin red staining ofthe mineralized extracellular matrix. After 8 weeks, histological and histomorphometric evaluations of undecalcified resin sections (bone-implant contact BIC % and bone mineral index BMI) were performed using light microscopy and scanning electron microscopy. Results: Alizarin red staining in conjunction with gene expression results confirmed osteogenic differentiation. Histomorphometric assessment using scanning electron microscopy demonstrated improved BIC% and BMI near the treated surface compared with the untreated surface. Conclusions: The complex of differentiated grafted ADSCs and extracellular matrix and the macrodesign and microdesign of the implant can improve osseointegration in low-density bone. © 2016 Taylor & Francis.

Item Type: Article
Additional Information: cited By 0
Subjects: Biochemistry, Genetics and Molecular Biology
Divisions: Faculty of Medicine > Basic Sciences > Department of Anatomy
Depositing User: editor . 2
Date Deposited: 08 Mar 2017 22:08
Last Modified: 08 Mar 2017 22:08
URI: http://eprints.kaums.ac.ir/id/eprint/191

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