Expression of nestin and nerve growth factors in adipose-derived mesenchymal stem cells

Ghorbanian, M.T. and Haji-Ghasem-Kashani, M. and Hossein-Pour, L. and Mirzaiyan, L. (2011) Expression of nestin and nerve growth factors in adipose-derived mesenchymal stem cells. Feyz Journal of Kashan University of Medical Sciences, 15.

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Background: Adipose tissue represents an accessible source of mesenchymal stem cells (Adipose tissue derived mesenchymal stem cells-ADSCs). ADSCs could be differentiated into various mesenchymal stem cells (e.g. chondrocytes, adipocytes, osteoblasts, and neuronal lineages). Neurotrophic factors are diffusible polypeptides that have a critical role in survival, proliferation and differentiation of stem cells. This study aimed to evaluate the proliferative capacity and the expression of some neurotrophic factors such as brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF), glial cell-derived neurotrophic factor (GDNF), neurotrophin-3 (NT-3), neurotrophin-4 (NT-4), nerve growth factor (NGF) and the expression of nestin in ADSCs. Materials and Methods: Rat ADSCs were isolated from the subcutaneous adipose tissue using mechanical and enzymatic digestion. These cells were cultured in αMEM supplemented with 10 fetal bovine serum. ADSCs of the third passage were evaluated by immunocytochemistry to detect the cell surface markers of CD90 and nestin. Reverse transcription-polymerase chain reaction (RT-PCR) was used to study the expression of the above-mentioned neurotrophic factors in the ADSCs. Moreover, adipogenic and chondrogenic differentiation of ADSCs were induced in-vitro.Results: The assessment of ADSCs identity and purity showed that 90 and 80 of the stem cells were positive for CD90 marker and nestin, respectively. According to RT-PCR results, the above-mentioned neurotrophic factors were expressed in these stem cells. Furthermore, a small number of cells were positive for cresyl violet staining. Conclusion: Adipose tissue contains a stem cell population that seems to be a good multipotential cell candidate for cell replacement therapy.

Item Type: Article
Subjects: Medicine
Divisions: Feyz journal
Depositing User: ART . editor
Date Deposited: 10 May 2017 11:50
Last Modified: 28 May 2017 12:57

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