A molecular beacon-based real time PCR assay for quantitative detection of Toxoplasma gondii in rat

Norozi, R. and Dalimi-Asl, A. and Forozandeh-Moghadam, M. and Ghaffarifar, F. (2012) A molecular beacon-based real time PCR assay for quantitative detection of Toxoplasma gondii in rat. Feyz Journal of Kashan University of Medical Sciences, 16.

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Official URL: http://feyz.kaums.ac.ir/article-1-1551-en.html
DOI: UNSPECIFIED

Abstract

Background: Application of quantitative real time PCR has evolved as a sensitive, specific, and rapid method for the detection of Toxoplasma gondii (T. gondii). The present study aimed to evaluate the efficacy of real time PCR method, using B1 gene, for the diagnosis of toxoplasmosis in the experimentally infected rats. Materials and Methods: Parasites were cultured in peritoneal cavity of mice and then the DNA was extracted in tachyzoite stage. The B1 gene of T. gondii was amplified by PCR and detected by real time PCR method based on the molecular beacon probe. Finally, real time PCR was evaluated for the quantization of T. gondii in the blood of the experimentally infected rats. Results: The B1 gene of T. gondii which was successfully amplified by PCR yielded an amplicon with an approximate length of 116 bp. Using this gene was evaluated highly appropriate for the quantization of T. gondii by real time PCR method. Conclusion: Application of real time PCR method is shown to be highly efficient in terms of sensitivity and rapidity for the detection of B1 gene as well as the quantization of T. gondii in blood of rat.

Item Type: Article
Subjects: Medicine
Divisions: Feyz journal
Depositing User: ART . editor
Date Deposited: 09 May 2017 14:00
Last Modified: 25 May 2017 13:26
URI: http://eprints.kaums.ac.ir/id/eprint/1848

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