Determining the effect of trace elements (Lead and Zinc) on amylin Amyloidogenesis in vitro

Mirhashemi, S.M. and Bahmani, F. and Nejati, F. and Talebi, F. and Khalaji, F. and Mahdizade, M. (2013) Determining the effect of trace elements (Lead and Zinc) on amylin Amyloidogenesis in vitro. Feyz Journal of Kashan University of Medical Sciences, 16.

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Background: Type 2 diabetes mellitus can be defined as a conformational disease because in which a constituent beta cell protein, islet amyloid polypeptide (amylin), undergoes a change in tertiary structure followed by a self-association. In this research, Lead (Pb) and Zinc effects in the aggregation of the amylin was examined experimentally. Materials and Methods: Human amylin peptide and other chemicals were purchased from Sigma-Aldrich Company. Aggregation was induced by adding stock solution to modified Krebs-Hensleit buffer (pH: 7.4) to a final concentration of 0.4 μM and incubated at 37oC for 144h. Zinc (50 μM) and Pb (10 μM) was prepared in the peptide containing solution, separately. Also a mixture of trace elements and amylin with the same concentration was prepared. The zinc and Pb free solution was selected as control. To monitor the peptide precipitation Thioflavin T fluorescence assay was performed. Results: The results showed that zinc increased amylin aggregation by 12.31 % (P<0.05) and Pb enhanced aggregation potential by 23.17 % (P<0.05). A combination of Zn and Pb increased amylin aggregation by 0.24% compared to Pb group. Conclusion: Under the experimental conditions, Pb and Zinc separately have permissive effects on formation of beta-amyloid sheet. However, pb along with Zinc has no synergistic effect on aggregation of amylin.

Item Type: Article
Subjects: Nutrition
Divisions: Feyz journal
Depositing User: ART . editor
Date Deposited: 09 May 2017 12:57
Last Modified: 24 May 2017 02:32

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