Evaluation of trace metal (cd, cr, cu) - induced oxidative stress in presence of H2O2 on purified DNA strands break from nonpathogenic Escherichia coli

Bahavar, M. and Tarbali, N. and Einolahi, N. and Dashti, N. (2013) Evaluation of trace metal (cd, cr, cu) - induced oxidative stress in presence of H2O2 on purified DNA strands break from nonpathogenic Escherichia coli. Feyz Journal of Kashan University of Medical Sciences, 16.

Full text not available from this repository.
Official URL: http://feyz.kaums.ac.ir/article-1-1780-en.html
DOI: UNSPECIFIED

Abstract

Background: Studies on the interactions of metal ions and DNA suggests the significant effect of metals on DNA and its damage. This study was carried out to examine the effects of some trace metals including Cadmium, Chromium and Copper on E.coli (ptcc1399) DNA. Materials and Methods: Purified DNA from bacteria was incubated with different concentrations of metals (1-100µM) and H2O2 for 1 hour in 37cº. Then the damage on treated DNA was examined using the electrophoresis and spectrophotometric methods. To control the activity of metal ions, EDTA and catalase were used. Results: Increasing the concentrations of the mentioned metals and H2O2, did not alone cause damage to DNA, but treatment of DNA with a mixture of the metals and H2O2 in concentrations > 10 µM could lead to breaks in DNA. Furthermore, the addition of EDTA and catalase to the mixture prevented DNA damage. Conclusion: Since DNA damage has occurred only in the presence of metals and H2O2, there is a generation of free radicals by this mixture. Therefore, the main reason for this evidence probably shows the participation of the metals in fenton reaction.

Item Type: Article
Subjects: Immunology and Microbiology
Divisions: Feyz journal
Depositing User: ART . editor
Date Deposited: 08 May 2017 17:37
Last Modified: 24 May 2017 02:36
URI: http://eprints.kaums.ac.ir/id/eprint/1751

Actions (login required)

View Item View Item